Dec 17, 2018 · TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has very low acid sensitivity. TagRFP was derived from TurboRFP with the following mutations: K42R/K67R/A68T/I93V/F110L/N112D/I115L/N122R/R138L/R155E/H157R/Q159D/Y169H/H171I/S173N/F192V/H193Y/F194Y/M216V/K220R/R231K.

Nov 25, 2021 · 通常被命名为 红色荧光蛋白 RFP的探针如 DsRed 、TagRFP及 tdTomato ，实际上具有明显的橙色多于红色的发射谱。 不考虑颜色的指示，用标准的 四甲基罗丹明异硫氰酸脂 （TRITC）滤光片设备，橙色光谱型的蛋白在多种颜色，如蓝绿色、绿色和红色情景中更易于成像。

Monomeric derivative of red fluorescent protein from Entacmaea quadricolor. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. The 1-base overhangs produced by BmrI may be hard to ligate. Sticky ends from …

Apr 15, 2020 · We show that a new single-residue mutant, super-TagRFP (stagRFP) has nearly twice the molecular brightness of TagRFP-T and negligible photoactivation. stagRFP facilitates significant...

Jun 17, 2007 · Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high...

TagRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively.

Nov 15, 2021 · super-TagRFP is a basic (constitutively fluorescent) red fluorescent protein published in 2020, derived from Entacmaea quadricolor.

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RFP (TagRFP) is a fluorescent compound with an excitation peak at 555 nm and an emission peak at 583 nm. Other spectra of interest include: PE (phycoerythrin), and Texas Red. AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.

Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.