TALENs: a widely applicable technology for targeted genome …
2012年11月21日 · Recently, transcription activator-like effector nucleases (TALENs) have rapidly emerged as an alternative to ZFNs for genome editing and introducing targeted DSBs. …
TALENs: Customizable Molecular DNA Scissors for Genome
2013年6月20日 · Transcription activator-like effector nucleases (TALENs), consisting of an engineered specific (TALE) DNA binding domain and a Fok I cleavage domain, are newly …
TALE nickase mediates high efficient targeted transgene integration at ...
2014年3月28日 · In this study, we designed TALENs targeting the human rDNA locus, and introduced inactivating mutations into one of the two FokI domains to make TALENickases. In …
TALENs: customizable molecular DNA scissors for genome
2013年6月20日 · Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector …
专家点评Cell | 新工具TALEDs，实现线粒体基因组的A-G精准编辑
2020年，David R. Liu课题组和Joseph D. Mougous课题组鉴定出可以在双链DNA上进行C>U编辑的脱氨酶 DddA （Double-stranded DNA-specific cytidine deaminase），并将之与TALE相结 …
Broad specificity profiling of TALENs results in engineered …
Although transcription activator-like effector nucleases (TALENs) can be designed to cleave chosen DNA sequences, TALENs have activity against related off-target sequences. To better …
TALEN基因组编辑技术入门指南与原理介绍-赛默飞| Thermo Fisher …
Invitrogen™ GeneArt™ TALs来源于细菌 Xathomonas 产生的TAL效应子，其DNA结合结构域包含不同数目的氨基酸重复序列。 每个重复包含33-35个氨基酸并可识别一个单DNA碱基对。 …
Broad specificity profiling of TALENs results in engineered ... - Nature
2014年2月16日 · To better understand TALEN specificity, we profiled 30 unique TALENs with different target sites, array length and domain sequences for their abilities to cleave any of 10 …
TALENs: a widely applicable technology for targeted genome …
Recently, transcription activator-like effector nucleases (TALEN s) have rapidly emerged as an alternative to ZFNs for genome editing and introducing targeted DSBs. TALENs are similar to …
Transcription activator-like effector nuclease - Wikipedia
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made by fusing a TAL effector DNA …