CellLight™ Talin-GFP, BacMam 2.0, provides an easy way to label talin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Plasmid GFP-Talin (mTalin1) from Dr. Chinten James Lim's lab contains the insert Talin1 and is published in Chinten James Lim This plasmid is available through Addgene.

Plasmid GFP-Talin1 from Dr. Anna Huttenlocher's lab contains the insert Talin and is published in Nat Cell Biol. 2004 Oct . 6 (10):977-83. This plasmid is available through Addgene.

Image talin, a ubiquitous cytosolic protein found in high concentrations in focal adhesions in live cells with CellLIght Talin-GFP. This ready-to-use fluorescent protein-based reagent contains the human C-terminus of talin fused to emerald GFP

We show that alcohol inducible expression of GFP-mTalin in root hairs causes severe defects in actin organization, resulting in either the termination of growth, cell death, and/or changes in cell shape. Fluorescence recovery after photobleaching experiments demonstrate that the interaction of GFP-mTalin and actin filaments is highly dynamic.

CellLight 试剂可标记活细胞中的肌动蛋白Actin、微管蛋白Tubulin或踝蛋白Talin，使研究人员能够追踪细胞骨架动力学。 CellLight 荧光融合蛋白可与其他荧光探针在多重分析中一起标记活细胞或固定后细胞以进行共定位研究。

We have found that transient and stable expression of the talin actin-binding domain fused to the C-terminus of the green fluorescent protein (GFP-mTn) can visualize the actin cytoskeleton in different types of living plant cells without affecting cell morphology or function.

CellLight Talin-GFP (C10611, Figure 11.2.2) and CellLight Talin-RFP expression vectors generate autofluorescent proteins fused to the C-terminal actin-binding domain of human talin (CellLight reagents and their targeting sequences—Table 11.1) and incorporate all the generic advantages of BacMam 2.0 delivery technology (BacMam Gene Delivery ...

2025年2月26日 · Talin regulates the adhesion and migration of cells in part by promoting the affinity of integrins for extracellular matrix proteins, a process that in cells such as endothelial cells and platelets requires the direct interaction of talin with both the small GTPase Rap1 bound to GTP (Rap1–GTP) and the integrin β3 cytoplasmic tail.

Here, we find that the scaffold protein talin undergoes force-induced cleavage in early adhesions to produce the talin rod fragment that is needed for cell cycle progression. Expression of noncleavable talin blocks cell growth, adhesion maturation, proper mechanosensing, and the related property of EGF activation of motility.