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2020年11月1日 · Ammonia-oxidizing bacteria (AOB) of the betaproteobacterial genera Nitrosomonas and Nitrosospira are key nitrifying microorganisms in many natural and engineered ecosystems. Since many AOB remain uncultured, fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes has been …

2020年5月27日 · However, the established and widely used AOB-specific 16S rRNA-targeted FISH. 28 datasets than available today. Several of these probes cover their target AOB lineages. 30 probes that should detect different AOB lineages. Here, a set of new highly specific 16S rRNA- 33 phylogenetic clusters of betaproteobacterial AOB.

2020年8月14日 · The new probes were successfully applied to visualize and quantify AOB in activated sludge and biofilm samples from seven pilot- and full-scale wastewater treatment systems. Based on its improved target group coverage and specificity, the refined probe set will facilitate future in situ analyses of AOB.

2020年5月27日 · Here, a set of new highly specific 16S rRNA-targeted oligonucleotide probes was developed and experimentally evaluated that complements the existing probes and enables the specific detection and...

新探针已成功应用于可视化和量化来自七个中试和大型废水处理系统的活性污泥和生物膜样品中的AOB。 基于改进的目标群体覆盖范围和特异性，完善的探针组将有助于未来AOB的原位分析。 更新日期：2020-08-14 点击分享 查看原文 点击收藏 阅读更多本刊最新论文

2020年7月19日 · Therefore, AOB are routinely quantified with real-time PCR (qPCR) targeting ammonia monooxygenase alpha subunit (amoA) gene (Aakra et al., 2001), in both natural and engineered systems (Sharma et al., 2007; Orschler et al., 2019).

2006年3月8日 · In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems.

Helpful calculator to get the target AOB, speed and distance.

In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha clu …