Optimization of tris (2-carboxyethyl) phosphine reduction conditions ...
2019年5月1日 · In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris (2-carboxyethyl) phosphine (TCEP) for fast analysis of total biothiols. Disulfides were reduced with TCEP, and then, thiols were derivatized with the fluorogenic reagent, ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F).
Four-color DNA sequencing with 3′-O-modified nucleotide …
2008年7月8日 · The 3′- O -azidomethyl group on the DNA extension product generated by incorporating each of the NRTs is efficiently removed by the Staudinger reaction by using aqueous Tris (2-carboxyethyl) phosphine (TCEP) solution (28, 29) followed by hydrolysis to yield a free 3′-OH group for elongating the DNA chain in subsequent cycles of the hybrid ...
tris(2-Carboxyethyl)phosphine (TCEP) for the Reduction of …
2003年10月1日 · The thermally activated forms of both compounds (1´-N3 and 2´-NO2) show fast response time, excellent selectivity and sensitivity for the detection of H2S under physiological conditions (HEPES ...
Quantitative analysis of tris(2-carboxyethyl)phosphine by anion ...
2012年2月5日 · In this paper, we discuss a sensitive high-performance liquid chromatography (HPLC) method equipped with an evaporative light scattering detector (ELSD) for the determination of TCEP in pharmaceutical samples containing …
DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide ...
Treatment with TCEP efficiently removes the azidomethyl group from the DNA extension product, yielding a free 3′-OH group for continuous sequencing. Meanwhile, the strong and distinct azido peak in Raman spectra makes it an excellent reporter group …
TCEP_百度百科
TCEP是一种非常有效的硫醇类还原剂，广泛用作蛋白质化学及蛋白质组学研究中二硫键的定量还原剂。 该试剂在水溶液中的稳定性和溶解性都很好。
A Tris (2-Carboxyethyl) Phosphine (TCEP) Related ... - ScienceDirect
2010年5月1日 · Introduced in the late 1980s as a reducing reagent, Tris (2-carboxyethyl) phosphine (TCEP) has now become one of the most widely used protein reductants. To date, only a few studies on its side reactions have been published.
Technology│点击化学试剂 - 知乎
第一代叠氮点击化学是通过叠氮 (N3) 与炔烃（如炔丙基）在铜催化剂存在下反应形成稳定的三唑键而实现的。 第二代叠氮化物点击化学通过叠氮化物分子与结构受限的炔烃（如 DBCO（二苯并环辛炔）或 BCN（双环 [6.1.0] 壬炔）轴承分子的反应）无需使用铜。
THPP, TCEP——分子生物学巯基还原试剂-百灵威
2020年9月21日 · 三 (3-羟丙基)膦 (TPP或THPP)和三 (2-羧乙基)膦盐酸盐 (TCEP)可溶于水，用作中性巯基还原剂，还原性强于二硫苏糖醇 (DTT)。 THPP和TCEP还可用于固定化金属亲和色谱 (IMAC)，不会还原涉及的金属。
In Situ Quenching of Trialkylphosphine Reducing Agents Using …
2017年9月14日 · When tris (2-carboxyethyl)phosphine (TCEP) is used as the reductant, the reaction stoichiometry predicts that for each mol. of TCEP added, one interchain disulfide should be reduced, generating two free thiols for drug linkage.