In cultures of human retinal pigment epithelial (RPE) cells, inhibition of Na,K-ATPase by ouabain and K (+) depletion decreased transepithelial electrical resistance (TER) and increased...

Transepithelial resistance (TER) of iPSC-derived RPE cells on RPE cell-derived ECM and nitrite-modified RPE cell-derived ECM. Monolayer permeability was assessed by TER in polarized...

TER is used to measure the ion flow across the epithelial layer, increasing with time as a culture develops 7, 50 with a higher TER indicating higher confluence, strong polarization of the RPE monolayer, and tight junctions between cells, 7 all properties that are important to the RPE’s function as a barrier. 111 Typical TER for the living human...

人诱导多能干细胞 （hiPSC） 视网膜色素上皮 （RPE）移植是治疗RPE变性（如 年龄相关性黄斑变性）的一种有前景的细胞治疗方法。 然而，目前的RPE替代疗法面临重大挑战。 从hiPSC来源的细胞中选择不同的RPE需要一个繁琐的手工过程，尽管RPE细胞团的质量差异很大，但是没有有效的方法来区分功能性RPE片层和不适合移植的RPE细胞。 为了克服这些问题，我们开发了从hiPSC生成RPE细胞片和基于图像的评价方法。 我们发现，用六种信号通路抑制剂和 烟酰胺 …

2020年9月1日 · From a developmental perspective, the RPE is a neuroepithelium, derived from the outer layer of the optic cup. Functionally, the RPE acts as a secretory epithelium, a regulatory barrier, a professional phagocyte, and an antigen-presenting cell.

Transplantation of stem cell-derived retinal pigment epithelial (RPE) cells is considered a viable therapeutic option for age-related macular degeneration (AMD)....

Oxidative stress causes retinal pigment epithelium (RPE) cell dysfunction and is a major risk factor leading to the development of dry-type age-related macular degeneration. Taking pharmacological and genetic approaches, we address the mechanisms by which sublethal oxidative stress inhibits RPE cell phagocytosis.

Retinal pigment epithelial (RPE) cells function, in part, to provide a permeability barrier between retina and choroid and to enable vectorial transport between these layers. 1 Their highly specialized junctional complexes separate cell surface membrane proteins and lipids into apical and basolateral membrane domains. 1 The tight junction, which...

Object:Research the guinea pig myopia model induced by concave spherical lens, observe the transepithelial electrical resistance of cultured retinal pigment epithelial ()cells in vitro,Explore and estable the stable expression time interval of TER value from the monolayer barrier model of RPE cell.Methods:Take30guinea pigs of the same batch of2-week-old tri …

2020年9月1日 · ELISA and transepithelial resistance (TER) measurement are standard methods to evaluate the function of RPE sheets 17, but they are low-throughput and time-consuming....