TET-labeled oligonucleotides (DNA) as hybridization probes are useful for various in vivo and in vitro research/diagnostic applications, and for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes.

Fluorescent label TET™ is a single-isomer fluorescein dye designed for detection and analysis of labeled PCR products on ABI PRISM™ genetic analysis systems. TET™ is equally useful in applications historically employing isotopic labels. As such, it eliminates the hazard associated with handling, storage, and disposal of radioactive materials.

Protein is reacted with 4,4’-dithiodipyridine (4-PDS), then with trifluoroethylthio (TET). All cysteines on the protein are conjugated with TET, which contains a CF3 group. Detailed Protocol:

荧光标记 tet™ 是一种单一异构体荧光素染料，设计用于在 abi prism™ 基因分析系统上检测和分析标记的 pcr 产物。 TET™ 在过去采用同位素标记的应用中同样有用。

Some of the most commonly employed fluorescent dyes are 6-carboxyfluorescein (FAM), its tetra- and hexachlorinated analogues (TET and HEX), and carboxy-X-rhodamine (ROX) (Figure 7). However, their properties are not ideal; they have broad emission spectra, are non-fluorescent at low pH, and are susceptible to photobleaching.

AAT Bioquest offers the most comprehensive product line of FRET building blocks for labeling oligonucleotides, including both the classic dyes and our outstanding Tide Fluor™ (donors) and Tide Quenchers™ (acceptors).

The light-absorbing properties of TAMRA, and spectral overlap with several commonly used fluorophores - including FAM, HEX, TET and JOE, make it useful as a FRET acceptor for the dual labeled FRET probes such as Molecular Beacons. TAMRA has been used extensively for real-time PCR and other molecular diagnostic applications.

TET can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes.

Here we show how we can attach a label, carrying a ¹⁹F atom, to protein tyrosines, through the use of a specific three component Mannich-type reaction.

Tet (Figure 1) is a tetrachloro derivative of fluorescein. This dye is often used in real-time PCR assays, especially for multiplexing with FAM and Hex. TET-labeled oligonucleotides are also useful as primers for DNA sequencing or genotyping (microsatellite/STR analysis). Figure 1. Structure of 5'-Tet (R = sequence).