The Tet-Off system makes use of the tetracycline transactivator (tTA) protein, which is created by fusing one protein, TetR (tetracycline repressor), found in Escherichia coli bacteria, with the activation domain of another protein, VP16, found in the herpes simplex virus.

The Tet-controlled transactivator, Tet-Off Advanced, is a fusion protein derived from the E. coli Tet repressor protein, TetR, which is joined to three minimal transcription activation domains from the HSV VP16 pro-tein.

The initial system Gossen and Bujard developed is known as tetracycline off: in the presence of tetracycline, expression from a tet-inducible promoter is reduced.

The Tet-Off and Tet-On expression systems are binary transgenic systems in which expression from a target transgene is dependent on the activity of an inducible transcriptional activator.

Tet-On and Tet-Off brands are the highest-performing and most-cited inducible mammalian expression systems. Since the orignal first generation of Tet-On and Tet-Off Systems a series of improvements to the system have resulted in three distinct generations of the technology.

Tet-Off cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by removing doxycycline (Dox) from the culture medium.

Jan 14, 2020 · With the “Tet-Off” and “Tet-On” systems, the expression of a gene of interest, or even a short hairpin RNA, can be controlled by the presence (or absence) of tetracycline or one of its derivatives (such as doxycycline).

Following doxycycline removal, the Tet-Off transgenic system allows continuous transgene expression in the absence of drug. This would be advantageous for long-term studies where maintained transgene expression is desired and could enhance recombination efficiency in inducible LoxP/Cre systems.

‍ Tet-regulated mouse and rat models have a very strong track record of success (more than 7000 publications). genOway builds its new models based on past successes, maximizing model performance and reliability. Tet technology can be used …

The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox.