In this study, we have provided the clear evidence to support the upregulation of TFAM in glioblastoma cell lines and the tissue from glioma patients. This information will provide critical knowledge for better understanding the regulatory mechanism of cancer as well as a promising therapeutic strategy for brain cancer.

2024年6月1日 · Patient-derived GBM organoids was a pragmatic screening model to evaluate antineoplastic activity of lead compounds. Honatisine induced mitochondrial dysfunction via causing mitonuclear protein imbalance and lethal UPRmt. Mitochondrial transcription factor A (TFAM) might be a novel target to overcome chemoresistance in GBM.

From the study, we found that the combination therapy (TMZ 250uM+celecoxib 30uM) showed excellent inhibitory effect to the GBM, the LN229 and LN18, which were the TMZ resistant GBM cell lines. Our data suggest that the combination therapy may inhibits cell proliferation, increases apoptosis, and increases the autophagy on LN229 and LN18.

In their study, a Western blot analysis with an anti-TFAM antibody showed markedly increased protein expression of TFAM in GBM cell lines, especially U343-MG and U373-MG cells. Quantitative real-time PCR (qRT-PCR) showed elevated mRNA levels of TFAM in the U251-MG, U343-MG, and U373-MG GBM cell lines.

胶质母细胞瘤（gbm）是最可怕的颅内恶性肿瘤。 对天然化合物在 GBM 治疗中的潜在应用的系统探索已成为关键且富有成效的研究途径。 在本研究中，对 96 种二萜类化合物作为潜在抗肿瘤药物的库进行了系统评估。

2018年6月18日 · We show that AMPK inhibition reduces viability of patient-derived GBM stem cells (GSCs) and tumours. In stressed (exercised) skeletal muscle, AMPK is activated to cooperate with CREB1 (cAMP...

2018年5月9日 · Mitochondrial transcription factor A (TFAM) is a protein that maintains mitochondrial DNA (mtDNA) integrity, and alterations in its expression are associated with mitochondrial damage and cancer development.

Notably, honatisine exhibited remarkable efficacy in patient-derived primary and recurrent GBM strains. Subsequently, we subjected this compound to comprehensive scrutiny, encompassing GBM cultured spheres, GBM organoids (GBOs), TMZ-resistant GBM cell lines, and orthotopic xenograft mouse models of GBM cells.

POLG expression was related to decreased mtDNA copy number, and its overexpression associated with TFAM expression levels also have an impact on long-term survival among GBM patients, interpreted as a potential predictive factor for better prognosis.

In the hopes of blocking tumor growth, Franco et al. studied the effects of 3 mM melatonin on human GBM cells and observed a reduced expression of mitochondrial transcription factor A (TFAM), which is an essential protein that maintains mitochondrial DNA (mtDNA) integrity [65,66].