This unit provides background and a description of the "First-Generation" automated DNA sequencing technology. It also includes protocols for using the current Applied Biosystems (ABI) automated DNA sequencing machines.

The first automated DNA sequencer was the AB370A, introduced in 1986 by Applied Biosystems. The AB370A was able to sequence 96 samples simultaneously, 500 kilobases per day, and reaching read lengths up to 600 bases.

We review the drastic changes to DNA sequencing technology over the last 50 years. First-generation methods enabled sequencing of clonal DNA populations. The second-generation massively increased throughput by parallelizing many reactions. Third-generation methods allow direct sequencing of single DNA molecules. 1. Introduction. “...

First-generation DNA sequencing methods consist of 2 methods: Maxam-Gilbert (chemical degradation) and Sanger (chain termination) methods which are based on the amplification of template DNA and gel electrophoresis, emerged at approximately the same time, but the completion times differ from each other.

2016年1月1日 · We review the drastic changes to DNA sequencing technology over the last 50 years. First-generation methods enabled sequencing of clonal DNA populations. The second-generation massively increased throughput by parallelizing many reactions. Third-generation methods allow direct sequencing of single DNA molecules.

2011年10月1日 · This unit provides background and a description of the "First-Generation" automated DNA sequencing technology. It also includes protocols for using the current Applied Biosystems (ABI)...

In 1986, the first automated sequencer---abi prism 310 gene analyzer was developed by an American company---Pe Abi. And then Hood and Smith utilized fluorescently labeled dNTP for electrophoresis technology. Therefore, the first commercial automatic sequencer was born.

The first automated DNA sequencer, the ABI370, was launched in 1986, and the first sequencer using capillary electrophoresis, the ABI 3700, in 1998. New sequencing technologies, called next-generation sequencing (NGS), started to be commercialized in 2005 and are evolving rapidly.

Handling huge sequencing gels in the wee hours of the morning indicated a need for automation. Hood asked him to evaluate a project initiated by a former lab member, Henry Huang, who was looking to automate sequencing using the ultraviolet absorption of DNA. ?

As a graduate student at Stanford University in the early 1990s, Jonathan Eisen convinced a friend with access to one of the first automated DNA sequencers to run 10,000 base pairs for him. Sequencing Milestones.