In molecular biology, trans-activating CRISPR RNA (tracrRNA) is a small trans-encoded RNA. It was first discovered by Emmanuelle Charpentier in her study of the human pathogen Streptococcus pyogenes, a type of bacteria that causes harm to humanity. [1]

In this review, we describe the role of the tracrRNA in immune defense of CRISPR-Cas9 and beyond, non-traditional functions involving tracrRNAs, and how the tracrRNA has enabled an expanding set of CRISPR technologies.

2018年3月29日 · crRNA is a part of gRNA of CRISPR that is complementary to the target DNA, while tracrRNA is the second part of gRNA, which serves as a binding scaffold for the Cas nuclease.

2021年11月23日 · In this review, we describe the biology of the tracrRNA and how its ongoing characterization has garnered new insights into prokaryotic immune defense and enabled key technological advances. Keywords: RNA processing; RNase III; crystal structure; immune defense; sgRNA.

2016年12月29日 · Recent work suggests the tracrRNA plays a role in maintaining the Cas9 protein in an active form allowing it to be able to target DNA. It likely also contributes to the overall stability (and thus efficiency) of the CRISPR-Cas9 complex.

2024年8月8日 · Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and cleavage.

2022年4月11日 · By reprogramming the crRNA-tracrRNA hybridized sequence, we show that engineered crRNA-tracrRNA interactions can not only enable the design of orthogonal cellular computing devices but also...