Three-Dimensional (3D) Tumor Spheroid Invasion Assay - PMC
The three-dimensional (3D) tumor spheroid invasion assay described here provides a rapid, automatable invasion system using a highly reproducible, standardized method 12 in a 96-well …
Blood-brain-barrier spheroids as an in vitro screening ... - Nature
2017年6月6日 · Here the authors develop a self-assembling BBB spheroid model with superior performance to the standard transwell BBB model, and use their platform to identify cell …
Development of a Novel 3D Tumor-tissue Invasion Model for High ...
2018年8月29日 · Scratch and transwell assays are commonly used because of ease of use, although their geometry, artificial constraints, and general lack of ECM limit their physiologic …
Mechanical compression regulates tumor spheroid invasion into a …
A modified Transwell assay to apply static mechanical compression on tumor spheroids compatible with microscopic imaging at single-cell resolution. A commercial Transwell assay …
Towards a more objective and high-throughput spheroid invasion …
2024年12月28日 · Multicellular spheroids embedded in 3D hydrogels are prominent in vitro models for 3D cell invasion. Yet, quantification methods for spheroid cell invasion that are …
A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for …
2019年5月3日 · The present study demonstrates proof-of-concept of a three-dimensional (3D) model to study label-free BBB transport as well as the resulting brain tumor cytotoxicity by …
Spheroid Models: Getting Started and Culture Tips - Cell …
2020年6月9日 · The Corning® spheroid microplates to generate uniform 3D spheroids combined with Corning® HTS-Transwell inserts enable researchers to investigate immune cell homing, …
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
2021年11月5日 · The transwell assay can better model cell invasion when the well inserts are coated with ECM substrates but, only a single parameter, i.e. the appearance of cells on the …
Simplified in vitro 3D co-culture-based blood-brain barrier model …
2022年9月10日 · The 2D BBB models are solely comprised of brain endothelial cells. In such models, a monolayer of brain endothelial cells is seeded onto transwell, resulting in lack of …
LKB1 and STRADα Promote Epithelial Ovarian Cancer Spheroid …
2024年11月5日 · EOC metastasis relies on the formation of multicellular aggregates, called spheroids. Given that Liver Kinase B1 (LKB1) is required for EOC spheroid viability and LKB1 …