2005年8月4日 · Biochemical analysis of TRBP-containing complexes revealed the association of Dicer-TRBP with Argonaute 2 (Ago2), the catalytic engine of RISC. The physical association …

2005年6月22日 · In vitro reconstitution assays demonstrated that TRBP is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. Knockdown of TRBP …

2016年12月9日 · Here we show that TRBP is an integral cofactor for time-efficient Dicer processing in RNA-crowded environments. We competed for Dicer processing of pre-miRNA …

2015年2月5日 · In mammals, the RNA-induced silencing complex (RISC) performs miRNA-mediated gene silencing by targeting an Argonaute protein (e.g., Ago2) to repress mRNAs …

2013年3月8日 · These duplexes are exported to the cytoplasm, and their conversion to mature 22-nt miRNAs is mediated by a transactivation response (TAR) RNA binding protein …

2009年5月7日 · While Dicer and Ago2 are RNases, TRBP is the double-stranded RNA binding protein (dsRBP) that loads small interfering RNA into the RISC. TRBP binds directly to Dicer …

2005年9月1日 · Biochemical analysis of TRBP-containing complexes revealed the association of Dicer-TRBP with Argonaute 2 (Ago2), the catalytic engine of RISC. The physical association …

Here, we describe in detail methods for the expression and purification of recombinant Dicer, TRBP, and Ago2 and protocols for the assembly of RLCs and RLC subcomplexes. We also …

2021年12月6日 · 位于dsRBD2内部的KR螺旋模序负责RNA结合, 第3个结构域即C末端碱性结构域主要介导蛋白质间的相互作用。依据TRBP的结构基础, 我们构建了TRBP及其截短体TAB, …

TRBP is a protein with three double-stranded RNA-binding domains. Argonaute 2 is an RNase and is the catalytic centre of RISC. Dicer associates with TRBP and Argonaute 2 to facilitate …