Sigma-Aldrich offers Roche-10812846001, Tris hydrochloride (Tris HCL) for your research needs. Find product specific information including molecular weight, formula, CAS, MSDS, protocols and references.

The quick answer is that tris is a basic buffer, whereas tris HCl is the acidic buffer. Keep in mind, buffers are used to resist changes to pH. Even small concentrations of a strong acid or base, without a buffer, could significantly change pH.

Tris HCl or tris hydrochloride (also known as Trizma HCl) is a buffering agent (acidic buffer) commonly used in molecular biology and biochemical settings to adjust the pH of a solution or stabilize the pH.

Tris HCl (Tris Hydrochloride) is an effective buffer solution for physiologic pH of most organisms and is useful in electrophoresis of biological molecules.

2025年2月13日 · Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.

Tris-hcl hydrochloride | C4H13Cl2NO3 | CID 88484562 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

2024年1月5日 · Tris Hydrochloride is a versatile buffering agent used in various biochemical and molecular biology applications. Its advantages stem from its strong alkalinity, allowing for the preparation of a wide pH range (from acidic to alkaline) buffering systems using only Tris as the buffering agent.

Tris Base and Tris HCl are two forms of the same compound, Tris (hydroxymethyl)aminomethane. Tris Base is the deprotonated form, while Tris HCl is the hydrochloride salt form. Tris Base is a strong base with a high pH, making it useful in biological and biochemical applications as a buffer to maintain a stable pH environment.

Tris is one of the most frequently used buffers in cell and molecular biology experiments. It has a pKa value of 8.06 at 25°C and an effective pH range between 7.0 and 9.0, which perfectly corresponds to the physiological pH range.