TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

Explore the TrypLE enzyme, a trypsin-like formulation that’s gentle on cells and scalable for your needs.

TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

TrypLE™ is an animal origin-free recombinant enzyme alternative to porcine or bovine trypsin for the dissociation of attachment-dependent cell lines from plasticware. TrypLE™ cleaves peptide bonds on the C-terminal side of lysine and arginine but with greater specificity than native trypsin preparations due to the superior purity of TrypLE ...

As an alternative to trypsin, we offer Gibco ™ TrypLE Express Enzyme, a gentler, animal origin–free* dissociation reagent. TrypLE Express Enzyme is a recombinant protein that works

The results indicate that TrypLE Express Enzyme is just as effective as the trypsin/EDTA solution for dissociation of adherent primary cells in culture. Advantages of TrypLE Express reagents include: • Gentle on cells—dissociate strongly adherent cells while retaining cell surface markers • Animal origin–free (AOF)—TrypLE Express ...

A recombinant enzyme replacing animal trypsin for the dissociation of adherent mammalian cells from plastic. TrypLE™ Express is based on the same enzyme found in TrypLE™

2004年1月1日 · TrypLE ™ Express is a recombinant fungal trypsin-like protease, which has proven effective at dis-sociating many different attachment dependent mammalian cell lines.

Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a …

2022年5月16日 · TrypLE Express Enzyme shows remarkable enzyme stability. At 4˚C and at room temperature, the enzyme remains stable for more than 12 months. Studies have shown that even at 37˚C, TrypLE Express...