Cortical microtubule arrays undergo rotary movements in
2007年1月14日 · Here, Arabidopsis hypocotyl microtubules wershee labelled with AtEB1a–GFP (Arabidopsis microtubule end-binding protein 1a) or GFP–TUA6 (Arabidopsis α-tubulin 6) to …
An Atypical Tubulin Kinase Mediates Stress-Induced Microtubule ...
2013年10月21日 · To test whether tubulins phosphorylated by PHS1 polymerize to microtubules in vivo, we coexpressed GFP-TUA6 (which labeled α-tubulin) and mCherry-TUB6 (which …
Altered microtubule dynamics by expression of modified
2005年6月22日 · We have previously reported that, when GFP was fused to the N-terminus of the Arabidopsis α -tubulin 6 isoform (TUA6) and expressed constitutively under the control of the …
The IPGA1-ANGUSTIFOLIA module regulates microtubule …
2022年8月17日 · For live-confocal imaging of cortical microtubules, cotyledons stably expressing GFP-TUA6 (excitation at 488, emission 500–550 nm) and mCherry-TUA5 (excitation at 561 …
Independent and intrabundle dynamics of GFP-MBD- and GFP-TUA6 …
In quantitative terms, cortical microtubules of hypocotyl epidermal cells stably transformed with GFP-MBD or GFP-TUA6 exhibited alternating periods of growth succeeded by fast shrinkage …
TUA6 Tubulin/FtsZ family protein [Arabidopsis thaliana (thale …
2024年9月18日 · Encodes an alpha-tubulin isoform required for right handed helical growth. GFP-tagging of cell components reveals the dynamics of subcellular re-organization in response to …
Mechanical forces in plant tissue matrix orient cell divisions via ...
2024年5月20日 · To discard possible unspecific increases of GFP signal induced by the stretching, we used UBQ10::Venus-TUA6, which showed the same effect, and actin marker …
Overlapping Localization of MAP65-2, -6, and -7 in Arabidopsis ...
2023年10月24日 · While both MAP65-6 and MAP65-7 fusion proteins bound apparent microtubule bundles within the cortical arrays, not all GFP:TUA6 or mCherry:TUA6 …
Visualization of microtubules in living cells of transgenic
Microtubules (MTs) were visualized in living cells of several tissues in transgenic Arabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it with Agrobacterium …
Phytochrome interacting factor proteins regulate cytokinesis in
2021年5月11日 · Our findings reveal an organ-specific role of PIF proteins in regulating cytokinesis by BUB3.1 during apical hook development. To protect their newly formed fragile …