TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3] The assay relies on the use of terminal deoxynucleotidyl transferase (TdT), an enzyme that catalyzes attachment of deoxynucleotides, tagged with a fluorochrome or another ...

基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP (fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP Nick-End Labeling) 法检测细胞凋亡的原理。

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks indepen …

Understand the methods used for TUNEL staining (also known as the TUNEL assay), and review TUNEL staining kits. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, also called the TUNEL assay, detects the DNA breaks formed when DNA fragmentation occurs in the last phase of apoptosis.

2024年8月23日 · TUNEL is used to detect cells with massive amount of DNA damage, especially apoptotic cells, as DNA fragmentation is one step of the apoptotic death program. To perform the TUNEL assay, samples are first fixed, then solubilized.

2021年1月2日 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quanti …

Several variants of the methodology that is based on fluorochrome-labeling of 3'-OH termini of DNA strand breaks in situ with the use of exogenous terminal deoxynucleotidyl transferase (TdT), commonly defined as the TUNEL assay, have been developed by us.

TUNEL is used to detect cells with massive amount of DNA damage, especially apoptotic cells, as DNA fragmentation is one step of the apoptotic death program. To perform the TUNEL assay, samples are first fixed , then solubilized .

2022年3月4日 · TUNEL assay detects apoptotic cells through DNA strand cleavage and labeling with fluorescent dye. Proper cell fixation and permeabilization are crucial for accurate results. Two types of TUNEL stains: FITC-dUTP (sensitive) and PI-dUTP (photobleaching-resistant). Counterstaining with DAPI helps distinguish apoptotic nuclei.

TUNEL Assay is a technique that allows for the detection of both single and double strand DNA breaks, using fluorescent or light microscopy, and can be coupled with flow cytometry for high-throughput analysis of thousands of samples, ensuring accuracy and reproducibility.