Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2. Surprisingly, we found that Cas9, previously identified as the nuclease responsible for ultimate invader destruction, is …

2023年9月6日 · To reduce off-target risks, we perform a bioinformatic search and identify a high-fidelity Cas9 variant of the II-B subfamily from Parasutterella secunda (PsCas9). PsCas9 shows improved...

Plasmid pSpCas9 (BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8 (11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene.

Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus requires Cas1, Cas2, and Csn2. Surpris-thermophilus ingly, we found that Cas9, previously identified as the nuclease responsible for ultimate invader destruction, is …

Type II-A Cas9 has attracted significant attention since Streptococcus pyogenes Cas9 with the engineered single-guide RNA (sgRNA) has been developed as the revolutionary genome editing tool (24,28–30). The structure and function of type II …

Chaperone-mediated autophagy (CMA) is a highly specific lysosomal-dependent protein degradation pathway. A critical molecular component of CMA is the lysosome-associated membrane protein (LAMP) type 2A, which is required for substrate uptake by the lysosome. Defects in the CMA pathway have been asso …

pME-Cas9-T2A-GFP Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals and with a C-terminal GFP. Sequence Author : Zon Lab / Addgene #63155

2024年7月12日 · In type II-A CRISPR systems, Cas9 uses the guide RNA to cleave target DNA sequences in the genome of infecting phages, and the tracrRNA to bind the promoter of cas genes and repress their transcription.

6 天之前 · (B) Caco-2-ACE2 Cas9 cells from the panel 2A were infected with rSARS-CoV-2 Venus or Omicron BA.2 at an MOI of 0.1 or 1, fixed 24 h later and analysed by automated microscopy. Y-axis indicates ...

Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2. Surprisingly, we found that Cas9, previously identified as the nuclease responsible for ultimate invader destruction, is …