LY294002 could reduce the phosphorylation of Akt and GSK3β (P< 0.01, P< 0.05), and also decrease the DRD2 mRNA (P<0 .05). Conclusion: Risperidone can activate the Akt-GSK3β signaling pathway in the U251 cells, and PI3K is a common regulatory site in Akt-GSK3β signaling and D2 receptor gene expression.

The changes of miRNA expression profile in human glioblastome U251 cells after AKT small interfering RNA transfection were examined by a microarray, and confirmed by Northern blotting. Down-regulation of AKT expression by siRNA decreased the activity of …

2019年4月1日 · In this study, we elucidated the inhibitory effects and mechanisms of human bone marrow mesenchymal stem cells (hBMSCs) on human glioma U251 cells using a co-culture system in vitro. The anti-tumor activity of co-cultured hBMSCs was assessed by morphological changes, the MTT assay, and Hoechst 33258 staining.

In this study, we elucidated the inhibitory effects and mechanisms of human bone marrow mesenchymal stem cells (hBMSCs) on human glioma U251 cells using a co-culture system in vitro. The anti-tumor activity of co-cultured hBMSCs was assessed by morphological changes, the MTT assay, and Hoechst 33258 staining.

2014年1月31日 · Stably overexpressing SLC22A18 human glioma U251 cells were generated to investigate the effect of SLC22A18 on the sensitivity of cells to irradiation in vitro using clonogenic survival assay....

2024年1月15日 · PI3K/AKT/mTOR pathway participates in the TNTs formation upon nanomaterials exposure in U251 cells. Cells were pretreated with 10 μM LY294002, 10 μM Perifosine or 25 nM Rapamycin for 30 min and then exposed to nanomaterials.

2014年12月1日 · Human glioma cells (U251) were cultured in vitro. Cells without any treatment as control, Western blotting was used for measuring the expression of Akt (Thr308 and Ser473) and GSK3beta (Ser9)...

2023年12月5日 · Following the publication of the above article, a concerned reader drew to the Editor's attention that, regarding the western blots featured in Fig. 3B on p. 670, the bands featured in the U251 and U251-MC lanes for the miR-21 and U6 experiments appeared to be duplicates of each other.

2023年8月17日 · rSLURP-1 suppresses the activation of ERK, p38 MAPK, and AKT kinases, but not JNK, in U251 MG cells. We analyzed the effect of rSLURP-1 on the activity of the main intracellular signaling pathways in U251 MG cells mediating glioma progression and associated with α7 nAChR activity [41–43].

In the current studies, the effect of resveratrol on phosphoinositide kinase-3 (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway was examined in human U251 glioma cells. Resveratrol decreased both the expression and phosphorylation of Akt.