2024年1月31日 · We recently discovered that UBR4, an E3 ligase known for its role in the N-end rule pathway 9, helps degrade aggregation-prone nascent polypeptides 10. As mutations in UBR4 cause ataxia and...

The down-regulation of MVBs was validated by transient silencing of UBR4 with siRNA . Consistent with a previous report [ 32 ], transmission electron microscopy showed that immunogold labeled UBR4 molecules were deposited to MVBs in HEK293 cells stably expressing UBR4-V5 ( Fig 7B ), indicating that UBR4 is degraded along the endosome-lysosome ...

2023年10月27日 · Biochemical analyses uncover that both the ubiquitin ligase complex UBR4-KCMF1 and the acetyltransferase NatC recognize proteins bearing an unacetylated N-terminal methionine followed by a...

2013年2月19日 · UBR4 of the YS endoderm is associated with a tissue-specific autophagic pathway that mediates bulk lysosomal proteolysis of endocytosed maternal proteins into amino acids. In cultured cells, UBR4 subpopulation is degraded by autophagy through its starvation-induced association with cellular cargoes destined to autophagic double membrane structures.

Among the regulators uncovered by this screen, we have found that RNAi for the UBR, UBR4, increases myofiber size in Drosophila. Similarly, UBR4 siRNA and muscle-specific UBR4 knockout induce myofiber hypertrophy and increase muscle mass in mice.

2024年1月5日 · Here we identify and characterize a distinct E3 module within human UBR4 consisting of a ‘hemiRING’ zinc finger, a helical-rich UBR zinc-finger interacting (UZI) subdomain, and an N-terminal...

2018年9月10日 · Such an inhibition of DQ-BSA degradation was reproduced when UBR4 was transiently silenced using siRNA (Fig. 6A,B). These results show that the defect in EE biogenesis causes the failure to degrade endosomal protein cargos, …

According to another report, the depletion of UBR1, UBR2, UBR4, and UBR5 using small interfering RNA (siRNA) increased apoptosis in various cancer cells . A study of RIPK1, a caspase-8 target, showed that its ubiquitination promotes cell survival . On the other hand, RIPK1 cleavage by caspase-8 generates a pro-apoptotic C-terminal fragment ...

2020年7月6日 · We identified UBR4 and UBR5 as ubiquitin E3 ligases involved in HC ER-associated degradation. Knockdown of UBR4 and UBR5 resulted in intracellular accumulation, enhanced secretion, and reduced ubiquitination of HC. In concert with these E3 ligases, PDIA3 was shown to cleave ubiquitinated HC molecules to accelerate HC dislocation.

2024年12月25日 · UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4) is a Protein Coding gene. Diseases associated with UBR4 include Alternating Hemiplegia Of Childhood 2 and Long Qt Syndrome. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System.