23673-1-AP targets UCP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts.

Anti-UCP1 antibody (ab10983) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in mouse, rat samples. Anti-UCP1 antibody (ab10983) specifically detects UCP1 (UniProt ID: P25874; Molecular weight: 33kDa) and is sold in 50 µL selling sizes.

A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 seconds. Blocking/Dilution buffer: 5% NFDM/TBST. UCP1 is a specific marker for brown adipose tissue (BAT). It is expressed exclusively in BAT, but not in white adipose tissue. Negative control: Mouse white adipose tissue. (PMID: 2999153).

Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane.