2019年7月22日 · Single-molecule real-time imaging revealed that UV-DDB forms transient complexes with OGG1 or APE1, facilitating their dissociation from DNA. Furthermore, UV-DDB moves to sites of 8-oxoG...

2022年2月21日 · Using two different approaches to introduce 8-oxoG in cells, we show that DDB2 is recruited to 8-oxoG immediately after damage and colocalizes with 8-oxoG glycosylase (OGG1) at sites of repair....

This review first discusses biochemical studies demonstrating how UV-DDB stimulates human 8-oxoG glycosylase (OGG1), MUTYH, and apurinic/apyrimidinic (AP) endonuclease (APE1) to increase their turnover at damage sites.

In mammalian cells, the modified base is excised by the 8-oxoguanine DNA glycosylase (OGG1), initiating the base excision repair (BER) pathway. OGG1 confronts the massive challenge that is finding rare occurrences of 8-oxoG among a million-fold excess of normal guanines.

Recombinant Human N-Glycosylase/OGG1 是一种DNA修复酶，其作为一种修饰酶, 主要具有DNA糖基化酶活性和脱嘌呤/脱嘧啶（apurinic/apyrimidinic，AP）裂解酶活性两种活性。 N-糖基化酶活性能从双链DNA 上去除受损嘌呤碱基产生脱嘌呤 (AP) 位点，而 AP 裂解酶活性则能从 AP 位点的 3 处进行切割产生一个 5 磷酸和一个 3 磷酸-α，β-不饱和醛。 此外OGG1还能切割其它异常碱基对，比如与胞嘧啶配对的7,8-二羟基-8-氧代鸟嘌呤 (8-氧代鸟嘌呤), 与胞嘧啶配对的8-羟基 …

2023年6月15日 · Moreover, we have shown that UV-DDB can assist chromatin decompaction, facilitating access of OGG1 to 8-oxoguanine damage in telomeres. This review summarizes the biochemistry, single-molecule, and cell biology approaches that our group used to directly demonstrate the essential role of UV-DDB in BER.

2015年7月7日 · Here we report on the design and properties of fluorogenic probes that directly report on OGG1 (and bacterial homologue Fpg) activity in real time as the oxidized base is excised. The probes are short modified DNA oligomers containing fluorescent DNA bases and are designed to utilize the damaged DNA base itself as a fluorescence quencher.

UV-DDB is well known for recognizing UV photoproducts and initiating the global genome nucleotide excision repair pathway. The discovery that UV-DDB binds to 8-oxoG lesions and abasic sites, led to the novel finding that UV-DDB enhances OGG1-mediated excision of 8-oxoG, facilitating OGG1 enzymatic turnover by displacing it from the abasic site ...

Single-molecule real-time imaging revealed that UV-DDB forms transient complexes with OGG1 or APE1, facilitating their dissociation from DNA. Furthermore, UV-DDB moves to sites of 8-oxoG repair in cells, and UV-DDB depletion sensitizes cells to oxidative DNA damage.

2021年1月1日 · We investigated whether phloroglucinol (1,3,5-trihydroxybenzene), by enhancing the expression and activity of 8-oxoG DNA glycosylase 1 (Ogg1), had an effect on the capacity of UVB-exposed human HaCaT keratinocytes to repair oxidative DNA damage.