UV-Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

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2022年8月28日 · Ultraviolet-visible (UV-vis) spectroscopy is used to obtain the absorbance spectra of a compound in solution or as a solid. What is actually being observed spectroscopically is the absorbance of light energy or electromagnetic radiation, which excites electrons from the ground state to the first singlet excited state of the compound or material.

2024年1月18日 · UV-Vis spectrophotometry is a sophisticated analytical method for measuring light absorption across the ultraviolet (UV) and visible (Vis) ranges of the electromagnetic spectrum that is applied in many different scientific domains.

Ultraviolet visible (UV-Vis) spectrophotometers use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm).

The presence of chromophores in a molecule is best documented by UV-Visible spectroscopy, but the failure of most instruments to provide absorption data for wavelengths below 200 nm makes the detection of isolated chromophores problematic.

Ultraviolet–visible (UV–visible) spectrophotometry is primarily a quantitative analytical technique concerned with the absorption of near-UV (180–390nm) or visible (390–780nm) radiation by chemical species in solution.

Ultraviolet–visible (UV–vis) spectroscopy encompasses absorption spectroscopy and reflectance spectroscopy in the UV–vis spectral region. Molecules containing π-electrons or non-bonding electrons (n-electrons) can absorb ultraviolet or visible light energy and be excited to higher anti-bonding molecular orbitals.

2022年7月14日 · UV spectroscopy involves the promotion of electrons (n, σ, π) from the ground state to a higher energy state. In U.V. spectroscopy, the excitation of the electron occurs in the range of 200-800 nm.

UV-vis spectroscopy is a cost-effective, simple, versatile, non-destructive, analytical technique suitable for a large spectrum of organic compounds and some inorganic species. As a function of wavelength, UV-vis spectrophotometers measure the absorption or transmission of light that passes through a medium.