碧云天生产的SUMO Protease (Small Ubiquitin-related Modifier Protease)，是一种重组表达的高度特异性识别SUMO化修饰或SUMO结构域，并水解SUMO羧基端(C端) x-Gly-Gly-x肽段中Gly-Gly后的肽键，从而去除SUMO化修饰或者去除SUMO融合表达蛋白中SUMO结构域的蛋白酶。

2023年11月1日 · Ulp1 is a protease which is naturally present in yeast and specifically cleaves Ubl proteins (ubiquitin-like proteins) such as SUMO1 and Smt3 from other proteins [4]. Ulp1 is a valuable tool in molecular biology for cleaving SUMO-tags from recombinant proteins.

SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle. Present with 377 molecules/cell in log phase SD medium.

2012年11月23日 · SUMO proteases are now known to fall into one of three distinct classes: the well-characterized UBL-specific protease (Ulp) and sentrin-specific protease (SENP) class; the desumoylating...

2011年10月28日 · Two yeast SUMO proteases, Ulp1 and Ulp2, contain a conserved cysteine protease domain that can remove the SUMO moiety from modified proteins. Recent evidence suggests that Ulp2, and its mammalian orthologs Susp1/SENP6 and SENP7, play a role in the removal of SUMO and SUMO chains from nuclear proteins [12 – 16].

The Ulp1 protease catalyzes two essential functions in the SUMO pathway: (1) processing of full-length SUMO to its mature form and (2) deconjugation of SUMO from targeted proteins. Selective reduction of the proteolytic reaction produced a covalent thiohemiacetal transition state complex between a Ulp1 C-terminal fragment and its cellular ...

SUMO proteases of the SENP/Ulp family are master regulators of both sumoylation and desumoylation and regulate SUMO homeostasis in eukaryotic cells. SUMO conjugates rapidly increase in response to cellular stress, including nutrient starvation, hypoxia, osmotic stress, DNA damage, heat shock, and other proteotoxic stressors.

2018年1月19日 · SUMO proteases of the SENP/Ulp family are master regulators of both sumoylation and desumoylation and regulate SUMO homeostasis in eukaryotic cells. SUMO conjugates rapidly increase in response to cellular stress, including nutrient starvation, hypoxia, osmotic stress, DNA damage, heat shock, and ot …

Our results demonstrated that Ulp1 was able to cleave Gly–Gly↓ motif-mutated substrates, indicating that the diglycine motif is not strictly required for Ulp1 cleavage.