A single-domain antibody is a peptide chain of about 110 amino acids long, comprising one variable domain (V H) of a heavy-chain antibody, or of a common IgG. These peptides have similar affinity to antigens as whole antibodies, but are more heat-resistant and stable towards detergents and high concentrations of urea.

深入了解纳米抗体VHH与单域抗体的制备过程、结构特点及优缺点，为您揭示生物医学领域的前沿技术，开启纳米抗体在疾病诊断与治疗的新篇章。

2023年5月24日 · 同型二聚体蛋白的H链在其N端区域包含一个专用可变域，称为纳米抗体（VHH），用于与其同源抗原缔合。 HCAb中的VHH与常规抗体的Fab片段（抗原结合片段）的结构和功能等效（图1）。 骆驼科生物家族包括：

Architecture of homodimeric heavy‐chain antibodies (bottom left) with the Ag‐binding single‐domain VHH, enlarged on top; and classical heterotetrameric antibodies (bottom right) with the Ag‐binding variable fragments (comprising VH and VL domains) enlarged on top.

2017年11月21日 · Comparison of the VHH domain (nanobody) of a camelid heavy chain antibody with its VH-counterpart of a conventional antibody. The three complementarity determining regions (CDRs) of the antigen-binding paratope are indicated in red, the framework region is indicated in cyan (camelid VHH) and yellow (human VH and VL).

We use structural alignments to compare sequence and structure variation within the variable loops and framework regions of Nb VHH domains and Ab VH domains.

纳米抗体 (nanobody,Nb)， 即 重链单域抗体VHH (variable domain of heavy chain of heavy-chainantibody) ，该类抗体只包含一个重链可变区（VHH）和CH2，CH3区，相比于其他抗体，轻链天然缺失。 纳米抗体晶体直径2.5nm，长4nm，是自然存在的可以和抗原结合的最小片段。 纳米抗体和传统抗体的优劣对比. 纳米抗体序列的特异性. 纳米抗体VHH的表面大概只有10个氨基酸与人VH的不同，在FR2中存在有四个特异的氨基酸：普通抗体FR2中V37、G44、L45和W47这4个 …

Here, we review the structural and biophysical properties of VHH domains, highlight recent VHH-based therapeutics and diagnostics, and provide insight into VHH engineering that may pave the way to next-generation single domain antibody applications.

In the realm of biotechnology and medicine, the terms nanobody and VHH are often used interchangeably to describe single-domain antibodies. These small-format versions of traditional antibodies have grown as cornerstones of scientific innovation from diagnostics to …

2021年12月4日 · By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones.