Tumor necrosis factor (TNF-α) is an important clinically tested cytokine that could induce autoimmune diseases and inflammation. Therefore, the anti-TNF-α therapy strategy was developed and used therapeutically in various diseases, especially in the cytokine storm associated chimeric antigen receptor (CAR) T-cell therapy and antiviral therapy.

2 天之前 · Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-α-specific nanobody.

2021年5月1日 · Nanobodies are the variable domains of camelid heavy chain antibodies (VHH). A new method was built to design humanized framework with high solubility using bioinformatics tools. The constructed library was of high quality and used to screen anti-TNF-a Nanobodies.

The variable domain of the heavy chain of the heavy chain antibodies (VHHs), also referred to as nanobodies, are single-domain antibody fragments derived from heavy chain antibodies found in Camelidae [1]. VHHs consist of four frame regions (FR1-4) and three complementarity-determining regions (CDR1-3) (Fig. 2.1) [2].

2024年4月27日 · In this study, we constructed a phage display library comprising single-domain antibodies (sdAbs; or “VHHs”), known for their small size and remarkable stability, using a total of 1.6 × 10 9 lymphocytes collected from 20 different alpacas, resulting in …

2016年12月1日 · The VHHs are antigen-binding region/domain of camelid heavy chain antibodies (HCAb). They have many interesting biotechnological and biomedical properties due to their small size, high solubility and stability, and high affinity and specificity for their antigens. HCAb and classical IgGs are evolutionary related and share a common fold.

Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-α-specific nanobody

纳米抗体 (Nanobody or VHH)由3个高变区域的抗原互补决定区 (complementarity determiningregions,CDRs)和4个框架区域 (frameworkregions,FRs)组成, 其序列和空间结构与传统抗体在以下方面的特征。 传统抗体VH和VL通过疏水作用力来稳定结构并共同构成抗原结合区。 与之相比，VHH仅含有3个可变区，尽管与抗原结合界面的表面积减少，但其仍具有较高的稳定性与亲和力。 它主要通过以下方式来适应轻链的缺失： a.

2024年7月1日 · Based on PSSM analysis, the CDR3 of cAbBCII10 VHH framework was identified, and a set of amino acids for the substitution of each PSSM-CDR3 position was defined. Using the Rosetta design SwiftLib tool, the final repertoire was back-translated to a degenerate nucleotide sequence.

2021年2月8日 · Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-α-specific nanobody. Single-chain antibody gene therapy strategy based on high-throughput screening triggers sustained antiviral activity in the body.