Here we describe for the first time the productive in vitro infection of human retinal pigment epithelial cells by varicella-zoster virus (VZV), resulting in a typical cytopathic effect (CPE) that is characterized by enlarged cells with increased ...

A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus...

水痘是由水痘-带状疱疹病毒(Varicella-Zoster virus ,VZV)引发的世界性传染疾病。 为了研制疫苗和有效诊断水痘,本文研究了VZV疫苗株在Vero细胞中传代适应、制备VZV抗原工艺和ELISA方法。

Here we describe for the first time the productive in vitro infection of human retinal pigment epithelial cells by varicella-zoster virus (VZV), resulting in a typical cytopathic effect (CPE) that is characterized by enlarged cells with increased granularity.

In this study, we describe an alternate infectivity assay for the attenuated VZV (vOka/Merck) strain, based on the enumeration of infected cells 24 to 72 h postinfection by semiautomated capillary flow cytometry.

VZV CPE in H&V cells was apparent by day 3 to 4, with many large foci; MRC-5 and A-549 shell vial cultures demonstrated fewer and smaller foci that were difficult to see at day 5. Sensitivity for VZV detection by CPE was 100% for H & V cells, 82% for MRC-5 cells, and 70% for A-549 cells when atypical vesicular lesions were cultured.

结果Veto及Veto—E 传代细胞接种5～7 d后出现典型的VzV CPE； 2BS、HLF及其它原代细胞经盲传三代未见CPE，括取细胞做荧光染色也未见阳性细胞。 维普资讯 http://www.cqvip.com

Two to three days after VZV infection of all cell cultures at a multiplicity of infection (MOI) of 0.1, a multifocal cytopathic effect (CPE) developed. CPE was characterized by multinucleated cells and virus-specific intranuclear inclusions as determined by immunofluorescence and EM.

2024年6月5日 · 细胞病变效应（cpe）：vzv感染会引起典型的细胞肿胀和融合现象。 免疫荧光识别：使用单克隆抗体对细胞单层进行免疫荧光染色是鉴定vzv的首选方法。 病毒分离：由于结果等待时间较长，vzv的病毒分离在实际操作中并不常用。 血清学检测

Infection of human neurons in vitro with varicella-zoster virus (VZV) at low multiplicity of infection does not result in a cytopathic effect (CPE) within 14 days post-infection (dpi),...