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2020年5月6日 · The vacuolar membrane marker vac-rk CD3–975 and the pFGC-eYFP-MdSUT4 construct were individually transferred into Agrobacterium tumefaciens strain GV3101 (pMP90) using the heat shock method, spread onto agar plates, and incubated at 28 °C for 3 days. A single colony was picked, suspended in LB medium, and incubated at 28 °C overnight under ...

2022年4月5日 · To confirm tonoplast localization of MdMa13, the vacuolar marker, Vac-rk (CD3–975) was co-expressed with MdMa13-GFP fusion construct in A. thaliana mesophyll protoplasts (Fig. 2 b). The fluorescence of MdMa13-GFP was completely merged with that of the mCherry tonoplast marker ( Fig. 2 e).

Laser-scanning confocal fluorescence microscopy revealed that the mCherry fluorescence from the tonoplast marker Vac-rk CD3-975 was merged with the YPF fluorescence from MdSUT4.1-YFP. This result...

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2022年3月7日 · Specifically, CBL2/3-CIPK3/9/23/26 complexes not only activate vacuolar two-pore K + (TPK) channels to promote K + remobilization from the vacuolar store, enabling plant adaptation to low K environments (Tang et al., 2020b), but also co-regulate the sequestration and transport of Mg 2+ into vacuoles to protect plants from Mg toxicity (Mogami et ...

2015年11月26日 · Upon transient Agrobacterium mediated cotransformation of N. benthamiana epidermal peel cells with GFP::OsCCX2 and vacuolar marker, vac-rk, the GFP fluorescence of GFP::OsCCX2 is completely...

激光共聚焦荧光显微镜显示液泡膜标记vacr -rk CD3-975的mCherry荧光与MdSUT4.1-YFP的YPF荧光合并。 说明MdSUT4.1是液泡膜定位蛋白。 图4

2007年8月1日 · Name / Stock Number: vac-rk / CD3-975. View Order History. Add To Cart. Resource Type: plasmid. Availability: available. Donors: Andreas Nebenfuehr; Donation Date: 08/01/2007. Date Released: 08/01/2007. Description: Plant organelle marker tonoplast-mCherry marker, d35S promoter, binary plasmid pBIN20, kanamycin selection in plants.