2023年10月1日 · Viable but nonculturable (VBNC) cells are characterized by their loss of culturability, ability to maintain a nonculturable state, and potential to resuscitate. The formation of VBNC cells can occur under a wider range of stress conditions, many of which are moderate and long-term in nature.

Viable but nonculturable (VBNC) bacteria refers as to bacteria that are in a state of very low metabolic activity and do not divide, but are alive and have the ability to become culturable once resuscitated. [1] Bacteria in a VBNC state cannot grow on standard growth media, though flow cytometry can measure the viability of the bacteria. [1] .

Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques.

Viable but nonculturable (VBNC) cells are characterized by their loss of culturability, ability to maintain a nonculturable state, and potential to resuscitate. The formation of VBNC cells can occur under a wider range of stress conditions, many of …

Stimulated by a variety of environmental, biological, or chemical stimuli, VBNC cells may resuscitate and recover their cell division ability with an elevated metabolic level, as well as pathogenicity and cell morphology (Figure 1).

2024年10月21日 · In this present review, we mainly focus the VBNC state of Vibrios and other human bacterial pathogens. Exposure to several factors like nutrient depletion, temperature fluctuation, changes in salinity and oxidative stress, antibiotic and other chemical stress can induce the cells to VBNC state.

In this review, we focus on the issues associated with bacterial culturability, the diversity of bacteria entering the VBNC state, mechanisms of induction into the VBNC state, resuscitation factors of VBNC cells and implications of VBNC resuscitation stimuli for cultivating these otherwise uncultured microorganisms.

The study with S. aureus, VBNC cells preserved the enzyme activities related to alkaline phosphatase, acid phosphatase, esterase lipase C8, esterase C4, and naphthol-AS-BI-phosphohydrolase like normal cells (Liao et al. 2021).

2023年12月25日 · This review explores the concept of the VBNC state, providing insights into the beneficial bacteria known to employ this strategy. The investigation covers different chemical and physical factors that can induce the latency state, cell features, and gene expression observed in cells in the VBNC state.

2014年6月1日 · Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques.