Viable but nonculturable (VBNC) bacteria refers as to bacteria that are in a state of very low metabolic activity and do not divide, but are alive and have the ability to become culturable once resuscitated.

2024年10月21日 · The availability of micro and macronutrients in the environment is a critical factor influencing the VBNC state. The depletion of nutrients allows the cells to enter the VBNC state as a survival mechanism, reducing the cell metabolism and conserving energy to …

2016年5月4日 · VBNC state in bacteria is characterized as living cells that are unable to grow on routine media on which they normally grow, have low levels of metabolic activity and become culturable when resuscitated. VBNC cells have many differences from the viable culturable cells.

2018年8月1日 · 活的但非可培养 (viable but non-culturable，VBNC)状态是非芽孢形成菌抵御不良环境的生存机制 [1]。 VBNC状态广泛存在于已培养和未可培养的微生物中，它是在特定条件下，微生物不可分离培养的一种状态。 据报道，99%以上的微生物因处于VBNC状态而无法分离培养。

2014年6月1日 · Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques.

2023年12月25日 · This review explores the concept of the VBNC state, providing insights into the beneficial bacteria known to employ this strategy. The investigation covers different chemical and physical factors that can induce the latency state, cell features, and gene expression observed in cells in the VBNC state.

In this review, we focus on the issues associated with bacterial culturability, the diversity of bacteria entering the VBNC state, mechanisms of induction into the VBNC state, resuscitation factors of VBNC cells and implications of VBNC resuscitation stimuli for cultivating these otherwise uncultured microorganisms.

2011年8月1日 · This article discusses approaches to researching VBNC bacteria to obtain knowledge that is lacking on their gene expression while in the VBNC state, and when they enter into and then recover from this state, when provided with the necessary nutrients and environmental conditions to support growth and cell division.

Results indicated that flow cytometry provides higher cell counts than classical microbiology (P < 0·05) in 73% of cases highlighting the possible presence of VBNC. Equivalent performances (repeatability and reproducibility) were obtained for both methods.

Viable but non-culturable (VBNC) bacteria represent a unique state of microbial life where cells are alive but cannot be cultured using conventional laboratory techniques.