Propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) is one of the promising methods for timely detection of VBNC pathogens in environmental samples. We developed and used a method for the first time to detection of VBNC P. aeruginosa in swimming pool water samples using a membrane filter (MF).

2021年2月18日 · Viable but nonculturable (VBNC) is a type of dormant form preserving the vitality of microorganisms, but it cannot be cultured by traditional laboratory techniques. The aim of this study is to develop a propidium monoazide-crossing priming amplification (PMA-CPA) method that can successfully detect S. enterica rapidly with high sensitivity and ...

2018年9月1日 · In this study, a method of real-time quantitative PCR coupled with propidium monoazide (PMA-qPCR) was developed and evaluated for the reliable quantification of the VBNC cells of V. parahaemolyticus. The samples of different ratios of viable and dead cells were detected by PMA-qPCR and qPCR to assess this method.

2020年6月1日 · PMA-ddPCR could accurately differentiate viable and VBNC cells from dead cells. PMA-ddPCR could detect low level viable E. coli O157:H7 in real food samples. Escherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world.

2020年4月1日 · To detect viable but non-culturable (VBNC) Vibrio parahaemolyticus (V. parahaemolyticus) with greater accuracy and sensitivity, we used an improved propidium monoazide (PMAxx) dye to eliminate dead cell interference in an IMS-PMAxx-real-time (quantitative) polymerase chain reaction (IMS-PMAxx-qPCR) assay.

基于叠氮溴化丙锭结合荧光定量PCR技术(propidium monoazide quantitative PCR,PMA-qPCR)能够利用活细胞的膜完整性区分活菌和死菌的原理,以副溶血弧菌ATCC 17802为对象,通过探讨PMA-qPCR方法中各项因素对活菌计数结果的影响,优化并建立了VBNC副溶血弧菌的定量检测方法。

2020年1月10日 · Here, we integrated propidium monoazide (PMA) with real-time polymerase chain reaction (qPCR) targeting the rpoB gene to detect and quantify Campylobacter jejuni in the VBNC state. First, we optimized the concentration of PMA (20 μM) that could significantly inhibit the amplification of dead cells by qPCR with no significant interference on ...

A propidium monoazide (PMA)-based quantitative real-time PCR (qPCR) method was established to detect the V. parahaemolyticus in the samples of different frozen periods and compared with the results of qPCR and plate culture methods.

在这项研究中，开发了一种实时定量 PCR 与单叠氮化丙啶 (PMA-qPCR) 结合的方法，并评估了对副溶血性弧菌 VBNC 细胞的可靠定量。 通过PMA-qPCR和qPCR检测不同比例的活细胞和死细胞的样品以评估该方法。 然后将该方法用于监测副溶血性弧菌的 VBNC 诱导过程，并通过荧光显微镜和共聚焦激光扫描显微镜 (CLSM) 观察、流式细胞术 (FCM) 分析对活细胞进行量化，同时确认细胞完整性和呼吸活性。 此外，验证了该方法的适用性，以检测具有不同副溶血性弧菌状态的人 …

2017年11月20日 · 摘要： 定量检测不同诱导条件下的活的非可培养(viable but non-culturable,VBNC)状态副溶血弧菌的变化情况,并对VBNC菌的呼吸活性进行分析.采用叠氮溴化丙锭(propidium monoazide,PMA)与荧光定量聚合酶链式反应(fluorescence quantitative polymerase chain reaction,qPCR)结合的技术(PMA-qPCR ...