Jul 3, 2024 · For each ECM marker (WFA, VCAN (V0), and NCAN), we detailedly analyzed its PNN morphology in three cortical regions: BA9, BA14r, and BA24. We classified PNNs into distinct morphological types based on the following parameters: 2D shape, the extent of envelopment of cellular processes, thickness of the envelope, and the staining intensity (Table ...

Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity. Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in …

Jul 3, 2024 · The transcriptome of late developmental and mature stages revealed a relatively stable expression of PNN-related transcripts (log2-transformed expression values: 6.5-8.5 for VCAN and 8.0-9.5 for NCAN). Finally, we propose a classification of PNNs that envelop GABAergic neurons in the human cortex.

Nov 1, 2024 · We characterized the expression of PNN components in mouse NAc using immunofluorescence and RNA in situ hybridization. We lowered brevican in NAc PVINs of adult mice using an intersectional viral and genetic method and quantified the effects on synaptic inputs to NAc PVINs and reward-motivated learning.

Nov 3, 2023 · The Sp7 targets Tnr, Tnc, and Versican (Vcan) play fundamental roles in constructing the PNN. 47, 62 In addition, Sp7 targets in oligodendrocytes include enzymes that are important for PNN formation, including disintegrin and metalloproteinase with thrombospondin motifs 4 (Adamts4) and sulfotransferases Chst1 and Chst11. 4, 63, 64 In line with ...

May 4, 2022 · Chondroitin sulphate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the central nervous system (CNS) and can exert effects on cell adhesion, motility,...

Nov 3, 2022 · This study aimed to investigate the effect of perineuronal net (PNN) and neurocan (NCAN) on spinal inhibitory parvalbumin interneuron (PV‐IN), and the mechanism of electroacupuncture (EA) in promoting spinal cord injury (SCI) repair through neurocan in PNN.

The transcriptome of late developmental and mature stages revealed a relatively stable expression of PNN-related transcripts (log2-transformed expression values: 6.5–8.5 for VCAN and 8.0–9.5 for NCAN). Finally, we propose a classication of PNNs that envelop GABAergic neurons in the human cortex.

Brain-derived neurotrophic factor (BDNF) increases chondroitin sulfate proteoglycan (CSPG) (Bcan, Ncan, and Vcan) mRNA expression in a JNK-dependent manner and enhances the maturity of cortical neurons in culture. mRNA expression of key (a) perineuronal net (PNN) components (CSPGs, hyaluronan synthases [Has1-3], Sema3a) in cortical neuronal ...

Remarkably, we discovered at least two types of molecularly distinct Aggrecan-rich PNN populations in vLGN, exhibiting non-overlapping spatial, temporal, and cell-type specific expression patterns. The mechanisms responsible for the formation of these two populations of PNNs also differ as the formation of Cat315 + PNNs (but not WFA + PNNs ...