2020年10月16日 · The non-structural protein 2 (nsP2) of alphavirus Venezuelan equine encephalitis virus (VEEV) is a cysteine protease that is responsible for processing of the viral non-structural polyprotein and is an important drug target owing to the clinical relevance of VEEV. In this study we designed two recom …

In this work, an acrylate and vinyl sulfone-based chemical series was investigated as promising starting scaffolds against VEEV and as inhibitors of the cysteine protease domain of VEEV’s nonstructural protein 2 (nsP2). Primary screen and dose response studies were performed to evaluate the potency and cytotoxicity of the compounds.

2020年10月16日 · The non-structural protein 2 (nsP2) of alphavirus Venezuelan equine encephalitis virus (VEEV) is a cysteine protease that is responsible for processing of the viral non-structural polyprotein and is an important drug target owing to the clinical relevance of VEEV.

In this study, we examined the evolution process of one of the alphaviruses, Venezuelan equine encephalitis virus (VEEV), to understand its adaptation mechanism to the inefficient packaging of the viral genome in response to serial mutations introduced into the capsid protein.

2015年11月26日 · The Venezuelan equine encephalitis virus (VEEV) nonstructural protein 2 (nsP2) cysteine protease (EC 3.4.22.-) is essential for viral replication and is involved in the cytopathic effects (CPE) of the virus.

2016年9月1日 · Our data suggest VEEV nsP2 induced translation shutoff in IFN-primed cells early after infection is an important factor in the resistance phenotype of VEEV by decreasing levels of pre-existing ISG's and reducing production of new ISG molecules, which engenders a cellular environment permissive to viral replication.

2007年6月5日 · The results of this study suggest a direct interaction of the amino terminal domain of VEEV nsP2 with the 5′ end of the viral RNA and a possibility of applying a similar approach in studying the 5′-terminal promoter functioning in replication of other RNA-positive viruses.

2024年9月13日 · Using single-cell imaging techniques and VEEV replicons encoding green or red fluorescent proteins, we observed full SIE capacity in three hours. Transient expression of VEEV nsP3, but not...

2006年9月1日 · In pursuit of this goal, we report the crystal structure of the C-terminal region of VEEV nsP2 (nsP2pro). This structure consists of two domains: a novel cysteine protease domain, followed by a methyltransferase-like domain of unknown function.

The non-structural protein 2 (nsP2) of alphavirus Venezuelan equine encephalitis virus (VEEV) is a cysteine protease that is responsible for processing of the viral non-structural polyprotein and is an important drug target owing to the clinical relevance of VEEV.