Herein, we rationally engineered an IgG–VHH format bsAb based on a human antibody R15‐F7 and a humanized nanobody P14‐F8‐35, both with broad and potent in vitro and in vivo activities.

2022年10月27日 · Bispecific antibodies (bsAbs) represent a unique platform to increase antibody breadth and to reduce neutralization escape. Herein, a novel immunoglobulin G-variable …

Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody …

2020年2月11日 · Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a …

To study the impact and mechanism of V H replacement, we generated mice in which antibody production depends on the replacement of a nonproductive V H D H J H rearrangement …

2024年3月27日 · The VHH molecules are the smallest naturally occurring antibody binding domains derived from heavy-chain only antibodies that can be found naturally in camelids and …

2020年1月15日 · For several years, we explored ways to substitute antibodies by nanobodies (also known as VHHs), the smallest recognizing part of camelids' heavy-chain antibodies: …

Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody …

2020年8月11日 · VHH antibodies are the isolated VHH domains of heavy-chain only IgG2 and IgG3 antibodies produced by camelid species, such as alpaca, llama, and camel. These …

Conventional antibodies prefer binding to the convex portion of the antigen, whereas VHHs prefer epitopes, such as crevices and clefts on the antigen. Therefore, developing candidates with …