Role of the Agrobacterium tumefaciens VirD2 protein in T …
1998年11月7日 · VirD2 is one of the key Agrobacterium tumefaciens proteins involved in T-DNA processing and transfer. In addition to its endonuclease domain, VirD2 contains a bipartite C …
Fusion of the Cas9 endonuclease and the VirD2 relaxase ... - Nature
2020年1月23日 · Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein combines the functions of …
农杆菌的T-DNA是如何整合进植物基因组的？ - 知乎
VirD2包含强大的C端 核定位信号 序列，能引导T复合物穿过植物细胞质，通过核孔 进入细胞核。 进入细胞核以后，T-DNA将 靶向植物基因组进行整合，整合前会从超T复合体中 去除蛋 …
微生物所研究团队开发CRISPR/Cas9和农杆菌毒力蛋白协同作用的 …
2023年4月25日 · 农杆菌介导的遗传转化技术在植物、真菌、酵母中广泛使用，其拓扑异构酶VirD1能够辅助核酸内切酶VirD2识别并切割T-DNA的特异核苷酸序列。 研究人员将Cas9 …
Interaction of the DNA modifying proteins VirD1 and VirD2 of
1998年8月8日 · Agrobacterium, a plant pathogen, is capable to stably transform the plant cell with a segment of its own DNA called T-DNA (transferred DNA). This process depends, among …
The VirD2 pilot protein of Agrobacterium -transferred DNA …
The bacterial virulence protein VirD2 plays an important role in nuclear import and chromosomal integration of Agrobacterium-transferred DNA in fungal, plant, animal, and human cells. Here …
Dimerization of VirD2 binding protein is essential for …
2014年3月13日 · The VirD2-binding protein (VBP) is a key cytoplasmic protein that recruits the VirD2-T-DNA complex to the VirD4-coupling protein (VirD4 CP) of the VirB/D4 T4SS apparatus.
VirD2 Protein of Agrobacterium tumefaciens Very Tightly Linked
The protein is shown to be the polypeptide VirD2, previously identified as a component of the T-DNA border endonuclease that initiates T-strand production. T-strands from an Agrobacterium …
Agrobacterium-mediated T-DNA transfer and integration by minimal VirD2 ...
1981年8月5日 · The VirD2 protein of Agrobacterium tumefaciens is essential for processing and transport of the T-DNA. It has at least three functional domains: a relaxase domain at the N …
Among the Ti-plasmid-encoded virulence (Vir) proteins that are required for T-strand formation and translocation to the recipient cell (Christie and Vogel 2000, Zhu et al. 2000), VirD2 is …