2023年5月1日 · The Verhoeff stain is one of the most commonly-used stains to visualize elastic tissue, as found in blood vessel walls, elastic cartilage, lungs, skin, bladder, and some ligaments. Elastic fibers are components of the extracellular matrix …

Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG), [1] is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 1908. [2]

2023年3月7日 · What is Verhoeff-van Gieson stain? Ira Van Gieson first described the Verhoeff-van Gieson (VVG) staining protocol in 1889 as a method of evaluating collagen fibers in neural tissue.

ELASTIC TISSUE FIBERS - VERHOEFF'S VAN GIESON (EVG) PURPOSE: This stain is useful in demonstrating atrophy of elastic tissue in cases of emphysema, and the thinning and loss of elastic fibers in arteriosclerosis, and other vascular diseases.

2024年1月26日 · Description: This method is used for identifying elastic fibers in tissues such as skin, aorta, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The elastic fibers will be stained blue-black and background will be stained yellow. Fixation: 10% formalin. Section: paraffin sections at 5 um.

VVG staining is quick and produces intense staining of elastic fibers. The VVG stain consists of two parts: Verhoeff stain consisting of an iron-hematoxylin stain that is specific for elastic fibers, and the van Gieson component, consisting of a counterstain that is specific for collagen.

Differentiate sections (regressive stain) in 2% ferric chloride for 1 minute and then check microscopically. The elastic fibers and nuclei should be black, and background should appear ‘dirty’.

The Verhoeff-Van Gieson (VVG) stain is a widely used histological staining technique that combines Verhoeff's elastic stain with the Van Gieson counterstain. This dual-stain method is particularly effective for differentiating and studying elastic fibers in tissue sections.

2015年12月26日 · VVG protocol.docx 1997 VERHOFF - VAN GIESON STAIN PROTOCOL (VVG) PRINCIPLE: The first step in the procedure is an overstaining of the tissue section with a soluble lake of hematoxylin-ferric chloride-iodine. Ferric chloride and iodine both serve mordant functions primarily and oxidizing functions secondarily. The latter characteristic

2023年5月1日 · The Verhoeff stain, also known as the Verhoeff-van Gieson stain, is a histological staining procedure developed by Frederick Herman Verhoeff in 1908. The Verhoeff stain is one of the most commonly-used stains to visualize elastic tissue, as found in blood vessel walls, elastic cartilage, lungs, skin …