2021年1月31日 · West Nile virus (WNV) nonstructural protein 3 (NS3) harbors the viral triphosphatase and helicase for viral RNA synthesis and, together with NS2B, constitutes the protease responsible for polyprotein processing. NS3 is a soluble protein, but it is localized to specialized compartments at the rough e …

This review describes the various roles of NS3, focussing on NS2B-NS3 protease and its function and regulation in WNV replication and infection. Current advances towards development of antiviral inhibitors of NS2B/NS3pro are examined along with obstacles to their development as an antiviral therapy.

2019年4月2日 · 国内外已有多个研究组开展了黄病毒ns2b-ns3蛋白酶的结构研究，针对目前已解析的黄病毒蛋白酶结构，本文综述了zikv、denv和wnv三种黄病毒ns2b-ns3pro蛋白酶及其抑制剂复合物的结构研究进展。

2023年8月18日 · The mutation from cytosine to uracil at position 5570 of the WNV genome, resulting in a proline to leucine substitution at residue 1825 (NS3 319), was selected for characterization because it was shown to be maintained exclusively in minority sequences, identified over multiple years, and mosquito biased. 22 Specifically, NS3 P319L was first ...

West Nile virus (WNV) has spread rapidly around the globe, efficiently crossing species from migrating birds into humans and other mammals. The viral protease NS2B-NS3 is important for WNV replication and recognizes dibasic substrate sequences common to other flaviviral proteases but different from most mammalian proteases.

这项研究描述了使用全长ns3的黄病毒蛋白酶活性的第一个生化特征。通过将西尼罗河病毒（wnv）ns2b（40）与全长wnv ns3融合来产生重组蛋白酶。该蛋白酶催化了两个自溶切割。纯化蛋白质之前，先切断ns2b / ns3连接。

2023年4月1日 · The main goal of our study was to obtain highly selective and specific substrates for monitoring WNV NS3 protease activity. Experiments with specific inhibitors confirmed that the optimized substrate is recognized by the WNV NS3 enzyme. In addition, we decided to check if the substrate is hydrolyzed by other proteases present in the test system.

2008年9月25日 · West Nile virus (WNV) has spread rapidly around the globe, efficiently crossing species from migrating birds into humans and other mammals. The viral protease NS2B-NS3 is important for WNV replication and recognizes dibasic substrate sequences common to other flaviviral proteases but different from …

2007年1月1日 · West Nile virus (WNV), a mosquito-borne member of Flaviviridae, is a human pathogen causing widespread disease for which there is no vaccine or chemotherapy. The two-component viral serine protease consists of a heterodimeric complex between the hydrophilic domain of the cofactor, NS2B (NS2BH) and the protease domain (NS3-pro).

2021年1月31日 · West Nile virus (WNV) nonstructural protein 3 (NS3) harbors the viral triphosphatase and helicase for viral RNA synthesis and, together with NS2B, constitutes the protease responsible for polyprotein processing.