2009年9月1日 · Recent studies in yeast have found that processing of DNA double-strand breaks (DSB) for recombination repair involves Sgs1 helicase. Human cells have five Sgs1 homologues with unknown selectivity and significance for repair of different DSB types.

In a previous report, we showed that WRN protein is recruited to laser-induced DNA double-strand break (DSB) sites during various stages of the cell cycle with similar intensities, supporting that WRN participates in both non-homologous end joining (NHEJ) and homologous recombination (HR).

2010年8月27日 · Here, we report that WRN is directly phosphorylated by ATR at multiple C‐terminal S/TQ residues. Suppression of ATR‐mediated phosphorylation of WRN prevents proper accumulation of WRN in nuclear foci, co‐localisation with RPA and causes breakage of …

2016年12月6日 · Here, we report that WRN regulates the pathway choice between classical (c)- and alternative (alt)-nonhomologous end joining (NHEJ) during DNA double-strand break (DSB) repair. It promotes...

In a previous report, we showed that WRN protein is recruited to laser‐induced DNA double‐strand break (DSB) sites during various stages of the cell cycle with similar intensities, supporting that WRN participates in both non‐homologous end joining (NHEJ) and homologous recombination (HR).

2010年9月15日 · Here, we report that WRN is directly phosphorylated by ATR at multiple C-terminal S/TQ residues. Suppression of ATR-mediated phosphorylation of WRN prevents proper accumulation of WRN in nuclear foci, co-localisation with RPA and …

Werner syndrome is a disorder characterized by genomic instability, aging pathologies and defective WRN, a RecQ-like helicase with exonuclease activity. We show that WRN interacts directly with the catalytic subunit of DNA-PK (DNA-PK CS), which inhibits both the helicase and exonuclease activities of WRN.

Werner syndrome protein (WRN) is a RecQ enzyme involved in the maintenance of genome integrity. Germline loss-of-function mutations in WRN led to premature aging and...

Recent studies in yeast have found that processing of DNA-double strand breaks (DSB) for recombination repair involves Sgs1 helicase. Human cells have five Sgs1 homologues with unknown selectivity and significance for repair of different DSB types.

ML216作为Bloom解旋酶（BLM）的抑制剂，也能够抑制人源成纤维细胞中WRN解旋酶的活性 [7]，本课题研究人员首次证明ML216通过激活p53/PUMA凋亡信号通路显著抑制小鼠微卫星不稳定肿瘤模型的生长，而恢复微卫星稳定性则完全抑制此信号通路和疗效（图2）。 为了进一步探索WRN抑制剂的临床转化潜力，研究人员分析了3个人源肿瘤组织来源移植瘤（PDX）模型，其中包括1个微卫星稳定PDX和2个微卫星不稳定PDXs。 令人惊喜的是，ML216在微卫星不稳 …