2021年6月3日 · ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product. However, ROX fluorescence is affected by anything else that would alter overall fluorescence readings ...

ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

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ROX（Passive Reference Dye）是一种惰性参比染料，在PCR反应中能被PCR仪检测到。 它不参与PCR反应，且荧光信号值是稳定的，不会随着PCR扩增发生改变。 qPCR实验过程中存在很多引起孔间差异的因素，例如不均匀的照明、孔与孔之间液滴、气泡等光学因素的影响（图1），反应体系的浓度差异。 此时，可以选择ROX作为阳性参比，对其他荧光信号进行归一化校正，以提高数据的精确度。 （图1） *在PCR过程中，如果使用的PCR反应板封膜质量不好，受到水蒸气的 …

ROX (carboxy-X-rhodamine) is used as a passive reference dye in ROX-dependent real-time PCR systems to normalize for differences in fluorescence levels that can occur due mainly to optical path variations among wells.

2019年5月20日 · ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

ROX is a fluorescent passive reference dye with a level of fluorescence that does not change during the PCR process. Normalization of qPCR data using ROX reduces the influence of well-to-well variability such as uneven illumination, slight variation in optics, and differences in the amount of condensation.

Rox is a corrupted anthropomorphic wolf, [1] one of the two leaders of the Forbidden Five alongside her brother Nokt, [2] and the Elemental Master of Fear. After enduring thousands of years of imprisonment in the Nether-Space alongside her peers, her soul escaped through the portal and took possession of Jordana during the Ritual of the Blood ...

2020年9月6日 · ROX is a passive reference dye which is not required in machines like Rotor-Gene Q from Qiagen because of the centrifugal rotary design.

Much like FAM™, VIC®, and SYBR® Green, ROX™ is a fluorescent molecule that the real-time instrument can detect when it's present in the reactions. But unlike those other dyes, ROX™ is a passive...