Kcat/Km就可以用来确定酶的最适底物，同一个酶对不同底物的Kcat/Km最大可相差100万倍。 当利用蛋白质工程对酶进行改造时，不同突变型的Kcat/Km也是需要测定的参数，用来表征酶催化效率的变化情况。

2025年1月13日 · From the tiny Rusty-spotted cat of Sri Lanka to the massive Siberian tiger of the Russian Far East, there are 41 species of wild cats in the world, and each of them is as beautiful as it is unique. Most of us know lions, tigers, jaguars and leopards, but what are all the other types of wild cats out there?

The wildcat is a species complex comprising two small wild cat species: the European wildcat (Felis silvestris) and the African wildcat (F. lybica).

RealKcat (trained on KinHub-27k) is the first-of-its kind catalytically aware kinetic predictor of enzymatic reactions, which is sensitive to mutations at the active center - and the first model to be able to predict zero kcat value if the catalytic apparatus of an enzyme is mutated.

2022年6月16日 · Here we provide a deep learning approach (DLKcat) for high-throughput kcat prediction for metabolic enzymes from any organism merely from substrate structures and protein sequences. DLKcat can...

The A135R mutant showed a 3-fold increase in catalytic efficiency (Km/Kcat) for homoglutamate compared with the wild type. ... Asymmetric Reductive Amination in Organocatalysis and Biocatalysis...

2016年11月19日 · Kcat, used to describe the limiting rate of any enzyme-catalyzed reaction at saturation. Most of the time K cat just equals K 2 (NOT the case when there are more reaction steps) I can find information about the calculation of the specificity constant (K …

The European wildcat (Felis silvestris) is a small wildcat species native to continental Europe, Great Britain, Turkey and the Caucasus. Its fur is brownish to grey with stripes on the forehead and on the sides and has a bushy tail with a black tip.

These mutants exhibited an elevated protease activity and, unlike the wild-type BepA, degraded LptD that is in the normal assembly pathway.

2021年7月16日 · For each of the variants, kcat is smaller than the wild type and km is larger for each of the variants than the wild type. So catalytic efficiency is less for the variants than the wild type. This indicates that both ions are essential for catalysis.