wpre是一段顺式作用的rna元件，为转录后调控序列，放在多聚腺苷酸化信号之前可显著增加mrna的表达水平和翻译效率，进而增强基因的表达，并且wpre插入病毒载体中还可以大大提升病毒包装的滴度。

Woodchuck Hepatitis Virus (WHV) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed, creates a tertiary structure enhancing expression. The sequence is commonly used in molecular biology to increase expression of …

1.WPRE不是polya的作用，是能够提高病毒产量的，只在病毒载体才会用。 病毒载体是用3LTR充当. 2.事实上，每个基因转录确实需要一个polyA来结尾提高mRNA的稳定性，但慢病毒由于不可以中间有polya，因此确实也牺牲了一部分RNA的稳定性，甚至有时还会造成两个启动子的相互干扰。 Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ.

2015年10月21日 · Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of …

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the posttranscriptional regulatory element of woodchuck hepatitis …

2007年6月28日 · WPRE is most effective when placed downstream of the transgene, proximal to the polyadenylation signal. We hypothesized that WPRE likely reduces viral mRNA readthrough transcription by...

The woodchuck hepatitis virus has a post-transcriptional regulatory element (WPRE) and our investigators found that it can substantially stimulate the expression of transgenes delivered by retroviral vectors, including spliced mRNAs and intronless transcripts.

WPRE can be shortened (to 247 bps), as demonstrated in neurons in vivo and in vitro, and it still offers sufficient transgene expression (Choi et al., 2014). In cultured human cells using lentiviral and AAV vectors, WPRE was found to increase CMV promoter driven transgene expression up to 8-fold (Loeb et al., 1999; Zufferey et al., 1999).

The vector has been optimized for vector titers and trans-gene expression in transduced cells by incorporating various cis-acting elements (EIAV Rev responsive element (RRE), Mason Pfizer monkey transport element (CTE), post-transcriptional regulatory element from woodchuck hepatitis virus (WPRE)) into the vector.

2005年12月15日 · Introduction of the post-transcriptional regulatory element (PRE) of woodchuck hepatitis virus (WHV) into the 3′ untranslated region of retroviral and lentiviral gene transfer vectors enhances both...