2021年7月8日 · The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). Under endoplasmic reticulum (ER) stress, unspliced Xbp1 mRNA is cleaved by the activated stress sensor IRE1α and converted to the mature form encoding spliced XBP1 (XBP1s). Translated XBP1s ...

2022年3月24日 · We show that Pumilio protects Drosophila Xbp1 against RIDD, without perturbing canonical Ire1-driven Xbp1 splicing. Furthermore, we provide evidence that Ire1 phosphorylates Pumilio under ER...

2025年1月9日 · Here, we uncover unfolded protein response stress sensor inositol-requiring enzyme-1α (IRE1α) signaling in hematopoietic stem and progenitor cells (HSPCs) as a safeguard against myeloid...

In response to increased demand on the protein folding capacity of the ER, UPR-mediated splicing of XBP1 mRNA yields the XBP1(S) transcriptional activator. We propose that XBP1(S) induces expression of unknown gene products that lead to an increase in CCT activity and a large increase in CPT activity, thereby augmenting synthesis of PtdCho.

Plasmid UAS-Xbp1-GFP from Dr. Herman Steller's lab contains the insert Xbp1 and is published in EMBO J. 2007 Jan 10;26(1):242-52. Epub 2006 Dec 14. This plasmid is available through Addgene.

2019年9月30日 · IRE1α catalyzes non-canonical splicing of X-box binding protein 1 (XBP1) mRNA into the constitutively active form XBP1s, which becomes the master UPR ER transcription factor controlling a wide...

2013年9月30日 · Here, we report that the XBP1 promoter activity itself is enhanced in secretory tissues of Drosophila, and it can be induced by excessive ER stress. Specifically, we developed a Drosophila XBP1 transcription reporter by placing dsRed under the control of …

2007年7月6日 · To identify tissue- and condition-specific XBP1 target genes, we analyzed five cell populations, including skeletal muscle myotubes, myotubes treated with 2-deoxy-D-glucose (2DG) to induce glucose deprivation, myotubes treated with insulin-like growth factor (IGF1) to induce anabolic stress, plasma cells (derived from a B cell hybridoma), and in...

Genetic interaction and biochemical analyses revealed that Ire1 regulates lipid mobilization by promoting Xbp1s-associated FoxO degradation and suppressing FoxO-dependent lipolytic programs. Our results demonstrate that Ire1 is a catabolic sensor and acts through the Xbp1s-FoxO axis to hamper the lipolytic response during chronic food deprivation.

2003年3月3日 · Here we show that UPR-induced splicing of XBP-1 by the transmembrane endonuclease IRE1 is required to restore production of immunoglobulin in XBP-1 −/− mouse B cells, providing an integral link...