2023年5月9日 · Here, we found that Th17 cell-inducing gut bacteria generated an unfolded protein response (UPR) in IECs. Furthermore, subtilase cytotoxin expression or genetic removal of X-box binding protein 1 (Xbp1) in IECs caused a UPR and increased Th17 cells, even in antibiotic-treated or germ-free conditions.

XBP1 also coordinates changes in cellular structure and function, as demonstrated by improvement of cell size, lysosome content, mitochondrial mass and function, ribosome number, and total protein synthesis 58. Under stressful conditions, XBP1 plays a critical role in promoting Th17 cell differentiation 59. IL-4 has been reported to promote the ...

ER stress inducers, tunicamycin, thapsigargin, and CPA, also enhance Th17 cell polarization; however, the treatment of stress inhibitor or XBP1 deficiency in naive T cells reduces Th17 cell differentiation and expression of IL-17 and RORγt. These results indicate that XBP1s may directly regulate the transcription of Rorc, which encodes RORγt.

Here, we found that Th17 cell-inducing gut bacteria generated an unfolded protein response (UPR) in IECs. Furthermore, subtilase cytotoxin expression or genetic removal of X-box binding protein 1 (Xbp1) in IECs caused a UPR and increased Th17 cells, even in antibiotic-treated or germ-free con-ditions.

Xbp1ΔIEC 或Duox2ΔIECXbp1ΔIEC 肠道内Th17细胞比Xbp1ΔIEC小鼠减少了。从而证明肠上皮细胞内质网应激通过上调 Duoxa2和Duox2基因表达促进Th17细胞的分化（Figure 4G-H）。 Figure3IEC ER stress 促进Th17细胞的分化依赖于Duox2/Duoxa2产生的ROS （图源：Duanet al.,Immunity,2023）

2023年6月30日 · In this study, we investigated the role of the UPR sensor IRE1 in TH17 cell function and neutrophilic airway inflammation. We found that IRE1 is induced in fungal asthma and is highly expressed in TH17 cells relative to naïve CD4 + T cells. Cytokine (e.g. IL-23) signals induce the IRE1-XBP1s axis in a JAK2-dependent manner.

2025年2月10日 · Assays for dual luciferase reporter and molecular docking indicated that XBP1s protein regulated the transcription of MAP3K2 by modulating promoter activity. The knockdown of MAP3K2 expression significantly inhibited the migration and EMT of gEECs.

2023年4月5日 · Analyses in Xbp1ΔIEC mice revealed that increased concentrations of purine metabolites (such as xanthine) contributed to IEC-ER stress T H 17 cell induction. In addition, the researchers showed...

Splicing of XBP1 mRNA was recognized in both intestinal compartments of IBD patients when compared to controls. Conclusions: In IBD patients, both intestinal compartments are involved in Th17 response, whereas, LP compartment plays a prominent role in …

2021年7月8日 · ER stress inducers, tunicamycin, thapsigargin, and CPA, also enhance Th17 cell polarization; however, the treatment of stress inhibitor or XBP1 deficiency in naive T cells reduces Th17 cell differentiation and expression of IL-17 and RORγt. These results indicate that XBP1s may directly regulate the transcription of Rorc, which encodes RORγt.