2019年7月22日 · For a more quantitative determination of XBP1 splicing activity, we measured the expression levels of total XBP1 (tXBP1: common region of s/uXBP1) and sXBP1 via real …

2021年9月7日 · Here, we report that CARM1-expressing ovarian cancer cells are selectively sensitive to inhibition of the IRE1α/XBP1s pathway. CARM1 regulates XBP1s target gene …

2016年4月7日 · IRE1α has been known to splice X-box binding protein 1 (XBP1) mRNA, which is induced by ATF6 under ER stress. This spliced XBP1 mRNA is translated into the active …

2020年7月20日 · Activation of the IRE1/XBP1s signaling arm of the unfolded protein response (UPR) is a promising strategy to correct defects in endoplasmic reticulum (ER) proteostasis …

We have now developed a simple and quantitative method to measure spliced human XBP1 by using quantitative real-time RT-PCR, and we show that the results obtained with this method …

2019年7月22日 · RT-qPCR was used to quantify the relative expression of the spliced version of XBP1 (XBP1s) by using specific pairs of primers for human alternatively spliced XBP1 and …

Conversion of unspliced XBP1 (uXBP1) to the spliced form (sXBP1) at the mRNA level was detected on agarose gels by semi-qPCR using a primer containing the cleavage site in the …

2011年10月31日 · In the present study, we have developed and validated a quantitative real-time RT-PCR method to detect the spliced form of XBP1 mRNA. Endoplasmic reticulum (ER) …

2019年7月22日 · For a more quantitative determination of XBP1 splicing activity, we measured the expression levels of total XBP1 (tXBP1: common region of s/uXBP1) and sXBP1 via real …

In the present study, we have developed and validated a quantitative real-time RT-PCR method to detect the spliced form of XBP1 mRNA. Endoplasmic reticulum (ER) stress is increasingly …