The Cell Proliferation Kit II (XTT) (Product No. 11465015001) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.

XTT is a two-reagent colorimetric assay used to assess cell viability as a function of cell number based on metabolic activity.

XTT is a negatively charged, tetrazolium salt that turns orange when it is reduced to a soluble formazan dye. This extracellular reduction is carried out by electron transport across the plasma membrane of a living cell.

The CyQUANT MTT assay is a well-established and popular cell viability assay, while the CyQUANT XTT assay provides a high dynamic range and low variability. In addition to improved performance, the CyQUANT XTT assay offers continuous measurement without the need for cell lysis or solubilization.

The XTT assay is an easy-to-use, optimized assay that contains the XTT reagent and an Electron Coupling Reagent. The XTT assay uses the cellular redox potential of metabolically active cells to reduce water-soluble XTT to a water-soluble orange-colored formazan.

Tetrazolium salts have been widely used as detection reagents for many years in histochemical localization studies and cell biology assays (1,2). The second generation tetrazolium dye, XTT, can be effectively used in cell proliferation, cytotoxicity, and apoptosis assays (2,3,4).

The CyQUANT™ XTT Cell Viability Assay is a complete and optimized kit for the detection of mammalian cell viability. The assay kit includes the XTT reagent (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2 H -Tetrazolium-5-Carboxanilide) and

The Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.

XTT Cell Viability Assay Protocol: easy to follow directions describing the step by step experimental procedure.

3 天之前 · The XTT Cell Viability Assay Kit is a colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells.