XbaI cleaves to leave a 5' CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or NheI. Blocked by overlapping dam methylation. Protocols, Manuals & Usage

Ligation and Recutting (Terminal Integrity) - After a 10-fold over-digestion of Adenovirus-2 DNA with XbaI, >95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16ºC. Of these ligated fragments, >95% can be recut with XbaI.

Thermo Scientific XbaI restriction enzyme recognizes T^CTAGA sites and cuts best at 37C in Tango buffer. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in on.

Generate new restriction sites by ligation of DNA fragments with compatible cohesive or blunt ends.

XbaI is a restriction enzyme isolated from the bacterium Xanthomonas badrii [1] ^ Biolabs, New England. "XbaI | NEB". www.neb.com. Retrieved 2016-05-08.

XbaI is blocked by overlapping dam methylation. If the XbaI recognition site is preceded by GA or followed by TC, the Dam methylase within most E. coli cloning strains will methylate the GATC site.

Prototype: XbaI Org #: 3059 Organism: Xanthomonas badrii DNA name: chromosome Org Comment: NCBI renamed this as Xanthomonas campestris pv. badrii Organism type: bacteria Complete genome Organism source: ATCC 11672 Growth Temperature: 26 ° Experimental Evidence: biochemistry Exhibits star activity Enzyme gene cloned Enzyme gene sequenced

Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.

NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free. An E. coli strain that carries the XbaI gene from Xanthomonas badrii (ATCC 11672).

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