2017年3月19日 · This new Xer complex is based on a single tyrosine recombinase called XerS (356 aa) that acts on an atypical 31 bp recombination site called difSL in the presence of dimers.

1997年8月29日 · Xer site-specific recombination functions in maintaining circular replicons in the monomeric state in Escherichia coli. Two recombinases of the bacteriophage λ integrase …

1999年10月15日 · We demonstrate that Xer recombination at plasmid dif sites occurs efficiently only when FtsK is present and under conditions that allow chromosomal dimer formation, …

克隆鲍曼不动杆菌xerC和xerD基因，并使用重组克隆来补充同源大肠杆菌突变体。 互补菌株支持质粒二聚体的解析，并且与大肠杆菌和肺炎克雷伯菌质粒的情况一样，当细胞在低渗透压生长 …

2017年3月20日 · In Escherichia coli and most bacteria, dimers are resolved to monomers by site-specific recombination, a process performed by two Chromosomally Encoded tyrosine …

2000年6月2日 · In Xer site-specific recombination, two related recombinases, XerC and XerD, belonging to the tyrosine recombinase family of site-specific recombinases, convert dimers of …

In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive …

1999年10月10日 · We propose that the chromosome dimer- and FtsK-dependent process that activates Xer recombination at plasmid dif also activates Xer recombination at chromosomal …

2001年9月7日 · We show that XerC or XerD variants lacking their N-terminal domains are active in recombination when combined with their wild-type partner. Nevertheless, the normal pattern …

2019年9月9日 · Xer-cise is a technique using antibiotic resistance cassettes flanked by dif sites allowing spontaneous and accurate excision from bacterial chromosomes with a high …