Function and Pathological Implications of Exon Junction Complex Factor Y14
2015年4月10日 · The exon-junction complex (EJC) is deposited on newly spliced mRNAs and coordinates several downstream steps of mRNA biogenesis. The EJC core protein, Y14, functions with its partners in nonsense-mediated mRNA decay and translational enhancement.
Function and Pathological Implications of Exon Junction Complex Factor Y14
The EJC core protein, Y14, functions with its partners in nonsense-mediated mRNA decay and translational enhancement. Y14 plays additional roles in mRNA metabolism, some of which are independent of the EJC, and it is also involved in other cellular processes.
The RNA-binding protein Y14 inhibits mRNA decapping and ... - PubMed
The exon-junction complex (EJC) deposited on a newly spliced mRNA plays an important role in subsequent mRNA metabolic events. Here we show that an EJC core heterodimer, Y14/Magoh, specifically associates with mRNA-degradation factors, including the mRNA-decapping complex and exoribonucleases, where …
The exon junction complex as a node of post-transcriptional
2015年12月16日 · The tetrameric core of the human exon junction complex (EJC), which binds to RNA, comprises the RNA helicase eukaryotic initiation factor 4A3 (eIF4A3), MAGOH, Y14 and metastatic lymph node 51...
Exon junction complex components Y14 and Mago still play a
2019年1月29日 · We report the identification of the core EJC components, Mago, Y14, and eIF4A3, in at least seven Saccharomycotina species, including Yarrowia lipolytica. Peripheral factors that join EJC,...
Comprehensive mapping of exon junction complex binding sites …
2023年11月7日 · The exon junction complex (EJC) is involved in most steps of the mRNA life cycle, ranging from splicing to nonsense-mediated mRNA decay (NMD). It is assembled by the splicing machinery onto mRNA in a sequence-independent manner. A fundamental open question is whether the EJC is deposited onto all exon‒exon junctions or only on a subset of them.
The Cellular EJC Interactome Reveals Higher-Order mRNP ... - Cell …
2012年11月9日 · Western blotting revealed two peaks containing the EJC core proteins eIF4AIII, Y14, and Magoh. These same two peaks were also observed upon gel filtration of RNase-A-treated cell lysates (data not shown), indicating that they were not …
NC | 停滞核糖体与外显子连接复合物的研究 - 生物学堂 - 新使生物 …
2024年5月27日 · EJC核心由eIF4A3、MAGOH和Y14组成。 团队将SmBiT和LgBiT分别融合到MAGOH的C端和Y14的N端，共同转染的细胞显示出高荧光素酶活性。 为了检测EJC组装，他们将SmBiT和LgBiT分别融合到eIF4A3和MAGOH的C端，结果也显示出高荧光素酶活性，表明EJC成功组装。 二. 影响EJC形成的突变也会削弱EJC-NanoBiT活性. 作将野生型和缺陷型MAGOH或eIF4A3突变体分别与对方共表达，突变体的荧光素酶活性显著降低，这表明荧光素酶活性反 …
Trypanosomes lack a canonical EJC but possess an UPF1 …
2025年3月7日 · Our data argue against the presence of a functional EJC in trypanosomes, but indicate that eIF4AIII adopted non-EJC related, essential functions, while Magoh and Y14 became redundant. Trypanosomes also possess homologues to the NMD proteins UPF1 and UPF2.
Transcriptome-wide modulation of splicing by the exon junction …
2014年12月5日 · The exon junction complex (EJC) is a dynamic multi-protein complex deposited onto nuclear spliced mRNAs upstream of exon-exon junctions. The four core proteins, eIF4A3, Magoh, Y14 and MLN51, are stably bound to mRNAs during their lifecycle, serving as a binding platform for other nuclear and cytoplasmic proteins.