Here, we provide experimental evidence that YAP removal confers ferroptosis resistance, whereas overexpression of YAP sensitizes cancer cells to ferroptosis. Furthermore, integrative analysis of transcriptome reveals S-phase kinase-associated protein 2 (SKP2), an E3 ubiquitin ligase, as a YAP direct target gene that regulates ferroptosis.

2018年6月11日 · Here, we report that YAP is subject to non-proteolytic, K63-linked polyubiquitination by the SCF SKP2 E3 ligase complex (SKP2), which is reversed by the deubiquitinase OTUD1.

2021年6月1日 · 此外，转录组的综合分析揭示了 S 期激酶相关蛋白 2 (SKP2)，一种 E3 泛素连接酶，作为调节铁死亡的 YAP 直接靶基因。 我们发现 YAP 敲低抑制了 SKP2 的表达。 重要的是，SKP2 的遗传和化学抑制作用有力地保护细胞免于铁死亡。 此外，敲除 YAP 或 SKP2 可消除erastin 诱导的铁死亡过程中的脂质过氧化。 总的来说，我们的结果表明，与 TAZ 相似，YAP 通过调节 SKP2 的表达是铁死亡的决定因素。 因此，我们的结果支持 Hippo 通路效应器与铁死亡 …

2023年4月8日 · We identify the E3 ubiquitin ligase Mind Bomb-2 (MIB2) as a FAT1-interacting protein mediating FAT1-induced YAP/TAZ ubiquitination and degradation. Loss of MIB2 expression in endothelial cells...

2014年12月1日 · We demonstrate that the E3 ubiquitin ligase SIAH2 stimulates YAP by destabilizing LATS2, a critical component of the Hippo pathway, in response to hypoxia. Loss of SIAH2 suppresses...

2022年12月15日 · In parallel, YAP underwent K63-linked polyubiquitination by SCF S-phase kinase-associated protein 2 (SKP2) E3 ubiquitin ligase, a Hippo-independent, nonproteolytic ubiquitination that enhances YAP nuclear trafficking and transcriptional activity in cancer cells.

2020年7月25日 · Cellular and molecular mechanisms in this study showed that PARK2 associated with YAP protein in the cytosol, promoted YAP ubiquitination and proteasome-dependent degradation in ESCC cells. Therefore, as a novel modulator for Hippo signaling, modulation of PARK2 activity or gene expression level …

我们将 E3 泛素连接酶 Mind Bomb-2 (MIB2) 鉴定为介导 FAT1 诱导的 YAP/TAZ 泛素化和降解的 FAT1 相互作用蛋白。 体外和体内内皮细胞中 MIB2 表达的缺失概括了 FAT1 耗竭的影响，并导致 YAP/TAZ 降解减少和 YAP/TAZ 信号传导增加。

Here, we provide experimental evidence that YAP removal confers ferroptosis resistance, while overexpression of YAP sensitizes cancer cells to ferroptosis. Furthermore, integrative analysis of transcriptome reveals S-phase kinase-associated protein 2 (SKP2), an E3 ubiquitin ligase, as a YAP direct target gene that regulates ferroptosis.

2020年6月1日 · LATS-mediated signaling crosstalk between hypoxia and YAP. (A) The E3 ubiquitin ligase SIAH2 and the scaffold protein zyxin direct LATS to be degraded via the ubiquitin–proteasome system, while TGFB strengthens this process.