The synthesis of QA by quinate/shikimate dehydrogenase (YdiB, ydiB) has been previously proposed; however, the precise role for this enzyme in the production of QA in engineered strains of E. coli for SA production remains unclear.

2003年5月23日 · Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity quinate/shikimate dehydrogenase that utilizes either NAD or NADP as a cofactor.

用于sh草酸（SA）生产的工程化大肠杆菌的培养导致奎尼酸（QA）和脱氢shi草酸（DHS）的合成，降低了SA的收率并损害了下游工艺。 先前已经提出了奎宁酸/ shi草酸脱氢酶（YdiB，ydiB）合成QA的方法。 然而，该酶在用于SA生产的大肠杆菌工程菌株中在QA产生中的确切作用尚不清楚。 我们报道了在大肠杆菌菌株PB12.SA22中ydiB的失活或过表达对分批发酵罐培养物中SA，QA和DHS生产的影响。 结果表明，相对于SA，相对于SA，ydiB的失活导致QA的摩尔产率降低 …

Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity quinate/shikimate dehydrogenase that utilizes either NAD or NADP as a cofactor.

2003年5月23日 · In this article, we report the crystal structure of the YdiB protein, which is one of the two shikimate 5-dehydrogenase orthologues in E. coli (12). It is common to find multiple orthologues of individual enzymes in the shikimate pathway in microorganisms.

莽草酸是芳香族氨基酸合成中的重要中间代谢物，可用于多种药物的化学合成．在代谢工程理论的指导下，构建积累莽草酸的高产菌株．通过构建敲除编码莽草酸激酶的基因aroL 与aroK的菌株阻断莽草酸的代谢；通过构建敲除编码奎尼酸／莽草酸脱氢酶的基因ydiB...

结果表明:与大肠杆菌野生型BW25113 (WT)相比, ydiB 基因敲除菌株 ( ydiB)仅对氨基糖苷类抗生素耐受,并且发现过表达菌株BW25113-PCA24N (ydiB)能恢复对庆大霉素的敏感性。 因此,证明 ydiB 基因是氨基糖苷类抗生素作用的关键基因,有望成为氨基糖苷类抗生素作用的新靶点。

Here, we report a study of Bacillus subtilis YdiB, which belongs to an uncharted class of bacterial P-loop ATPases. Precise deletion of the ydiB gene yielded a mutant with much reduced growth rate compared to the wild-type strain.

2003年5月23日 · We present here the 2.3-A crystal structure of the Escherichia coli YdiB protein, an orthologue of shikimate 5-dehydrogenase. This enzyme catalyzes the reduction of 3-dehydroshikimate to shikimate as part of the shikimate pathway, which is absent in mammals but required for the de novo synthesis of …

2016年11月18日 · We report the effect of the inactivation or the overexpression of ydiB in E. coli strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures.